Tailored oils
First Claim
1. An oleaginous microalgal cell of genus Prototheca, the cell comprising an active exogenous KASII (ketoacyl ACP synthase) gene encoding a protein having at least 95% sequence identity to the protein encoded by nucleotides 4039 to 5328 of SEQ ID NO:
- 95 and producing an oil comprising triglycerides, wherein the oil is enriched in stearic-oleic-stearic.
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Accused Products
Abstract
Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
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Citations
20 Claims
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1. An oleaginous microalgal cell of genus Prototheca, the cell comprising an active exogenous KASII (ketoacyl ACP synthase) gene encoding a protein having at least 95% sequence identity to the protein encoded by nucleotides 4039 to 5328 of SEQ ID NO:
- 95 and producing an oil comprising triglycerides, wherein the oil is enriched in stearic-oleic-stearic.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. An oleaginous microalgal cell of genus Prototheca, the cell comprising an exogenous gene encoding an active KAS II (ketoacyl ACP synthase) enzyme that has at least 95% sequence identity to the protein encoded by nucleotides 4039 to 5328 of SEQ ID NO:
- 95 and nucleic acids operable to reduce the expression of a stearoyl-ACP desaturase, the cell producing an oil comprising triglycerides, wherein the oil is enriched in triglycerides of the saturated-unsaturated-saturated type.
- View Dependent Claims (17, 18, 19, 20)
Specification