FcγRIIB specific antibodies and methods of use thereof
First Claim
1. A bispecific antibody comprising:
- (A) a first antigen binding polypeptide that selectively binds Fcγ
RIIB and comprises CDR1, CDR2 and CDR3 from an antibody heavy chain, and CDR1, CDR2 and CDR3 from an antibody light chain, wherein;
(1) said light chain CDR1 has a sequence of SEQ ID NO;
36, said light chain CDR2 has a sequence of SEQ ID NO;
37, and said light chain CDR3 has a sequence of SEQ ID NO;
38; and
(2) said heavy chain CDR1 has a sequence of SEQ ID NO;
27, said heavy chain CDR2 has a sequence of SEQ ID NO;
28, and said heavy chain CDR3 has a sequence of SEQ ID NO;
29,and said first antigen binding polypeptide has the binding characteristics of an antibody produced from a hybridoma cell line having ATCC accession number PTA-4592; and
(B) a second antigen binding polypeptide, that specifically binds an activating receptor.
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Accused Products
Abstract
The present invention relates to antibodies or fragments thereof that specifically bind FcγRIIB, particularly human FcγRIIB, more particularly the extracellular domain of FcγRIIB with greater affinity than said antibodies or fragments thereof bind FcγRIIA, particularly human FcγRIIA, and block the Fc binding site of FcγRIIB. The present invention also encompasses the use of an anti-FcγRIIB antibody or an antigen-binding fragment thereof, as a single agent therapy for the treatment, prevention, management, or amelioration of a cancer, preferably a B-cell malignancy, particularly, B-cell chronic lymphocytic leukemia or non-Hodgkin'"'"'s lymphoma, an autoimmune disorder, an inflammatory disorder, an IgE-mediated allergic disorder, or one or more symptoms thereof. The present invention also encompasses the use of an anti-FcγRIIB antibody or an antigen-binding fragment thereof, in combination with other cancer therapies. The present invention provides pharmaceutical compositions comprising an anti-FcγRIIB antibody or an antigen-binding fragment thereof, in amounts effective to prevent, treat, manage, or ameliorate a cancer, such as a B-cell malignancy, an autoimmune disorder, an inflammatory disorder, an IgE-mediated allergic disorder, or one or more symptoms thereof. The invention further provides methods of enhancing the therapeutic effect of therapeutic antibodies by administering the antibodies of the invention to enhance the effector function of the therapeutic antibodies. The invention also provides methods of enhancing efficacy of a vaccine composition by administering the antibodies of the invention with a vaccine composition. The invention further provides methods of treating cancer and/or regulating immune complex-mediated cell activation by administering the antibodies of the invention to enhance an immune response. The invention also provides methods of breaking tolerance to an antigen by administering an antigen-antibody complex and an antibody of the invention.
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Citations
25 Claims
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1. A bispecific antibody comprising:
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(A) a first antigen binding polypeptide that selectively binds Fcγ
RIIB and comprises CDR1, CDR2 and CDR3 from an antibody heavy chain, and CDR1, CDR2 and CDR3 from an antibody light chain, wherein;(1) said light chain CDR1 has a sequence of SEQ ID NO;
36, said light chain CDR2 has a sequence of SEQ ID NO;
37, and said light chain CDR3 has a sequence of SEQ ID NO;
38; and(2) said heavy chain CDR1 has a sequence of SEQ ID NO;
27, said heavy chain CDR2 has a sequence of SEQ ID NO;
28, and said heavy chain CDR3 has a sequence of SEQ ID NO;
29,and said first antigen binding polypeptide has the binding characteristics of an antibody produced from a hybridoma cell line having ATCC accession number PTA-4592; and (B) a second antigen binding polypeptide, that specifically binds an activating receptor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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Specification