Methods and apparatus for biomedical agent multi-dimension measuring and analysis

• US 8,947,662 B1
• Filed: 01/23/2013
• Issued: 02/03/2015
• Est. Priority Date: 01/26/2012
• Status: Expired due to Fees

• Current Assignee: Ann Rachel Yufa
• Original Assignee: Ann Rachel Yufa
• Inventors: Yufa, Ann Rachel, Yufa, Aleksandr Leybovich
• Primary Examiner(s): Chowdhury, Tarifur
• Assistant Examiner(s): Amara, Mohamed
• Application Number:
  US13/694,979
• Time in Patent Office: 741 Days
• Field of Search: 356/343, 356335-338
• US Class Current: 356/343
• CPC Class Codes: G01N 15/0205 by optical means
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2021/1734 Sequential different kinds of measurements; Combining two or more methods
• CPC Class Codes: G01N 21/53 within a flowing fluid, e.g. smoke
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 21/85 Investigating moving fluids or granular solids

Abstract

This invention, methods and apparatus for biomedical agent multi-dimension measuring and analysis, provide the multi-dimension measuring of the biomedical agents, cells, molecules, any and all explosive and hazardous agents, aerosol, airborne particles, liquid/fluid contaminations/particles, gas particles, etc. in order to recognize the multi-dimension size of the particles, select and sort them by their multi-dimension measurements, and count the particles in each sorted group. An improved apparatus for biomedical agent multi-dimension measuring and analysis comprises at least two light (laser) beam sources and at least two light detection means respectively located in the chamber. Also, the improved apparatus includes the amplifying, processing and control means providing distinguish and imaging of the particles/biomedical agents by their multi-dimensions, thereby, providing more precise agent grouping for analysis and counting.

Claims

1. A method for biomedical agent multi-dimension measuring and analysis comprising the steps of providing at least three light beam sources of a plurality of light beam sources, wherein a first light beam source provides a first light beam along a first light beam axis, a second light beam source provides a second light beam along a second light beam axis, and a third light beam source provides a third light beam along a third light beam axis, and wherein those three light beam axes are the axes of XYZ coordinate system;

directing said first light beam to a first light detection means through a biomedical agent monitoring region, wherein said first light detection means is located on said first light beam axis;

directing said second light beam to a second light detection means through said biomedical agent monitoring region, wherein said second light detection means is located on said second light beam axis;

directing said third light beam to a third light detection means through said biomedical agent monitoring region, wherein said third light detection means is located on said third light beam axis;

providing a flow of said biomedical agent along a biomedical agent flow axis through said biomedical agent monitoring region;

providing a first intersection of said biomedical agent flow axis with said first light beam axis at a first point on said biomedical agent flow axis, wherein said first point of said first intersection is located within said biomedical agent monitoring region on said first light beam axis between said first light beam source and said first light beam detection means located on said first light beam axis;

providing a second intersection of said biomedical agent flow axis with said second light beam axis at a second point on said biomedical agent flow axis, wherein said second point of said second intersection is located within said biomedical agent monitoring region on said second light beam axis between said second light beam source and said second light beam detection means located on said second light beam axis, and wherein a location of said second point on said biomedical agent flow axis is different from location of said first point on said biomedical agent flow axis;

providing a third intersection of said biomedical agent flow axis with said third light beam axis at a third point on said biomedical agent flow axis, wherein said third point of said third intersection is located within said biomedical agent monitoring region on said third light beam axis between said third light beam source and said third light beam detection means located on said third light beam axis, and wherein a location of said third point on said biomedical agent flow axis is different from location of said first point on said biomedical agent flow axis and from location of said second point on said biomedical agent flow axis;

providing a detection of a light, which is created during said first intersection, by said first light detection means, wherein said light is the light obstructed by said biomedical agent flowing through said first point located within said biomedical agent monitoring region, and wherein said first light detection means detects the obstructed light from said first light beam source along said first light beam axis which is perpendicular to said second light beam axis and to said third light beam axis;

providing a detection of a light, which is created during said second intersection, by said second light detection means, wherein said light is the light obstructed by said biomedical agent flowing through said second point located within said biomedical agent monitoring region, and wherein said second light detection means detects the obstructed light from said second light beam source along said second light beam axis which is perpendicular to said first light beam axis and to said third light beam axis;

providing a detection of a light, which is created during said third intersection, by said third light detection means, wherein said light is the light obstructed by said biomedical agent flowing through said third point located within said biomedical agent monitoring region, and wherein said third light detection means detects the obstructed light from said third light beam source along said third light beam axis which is perpendicular to said first light beam axis and to said second light beam axis;

providing by said first light detection means an output which is effectively indicative of a first dimension of said biomedical agent flowing through said first point;

providing by said second light detection means an output which is effectively indicative of a second dimension of said biomedical agent flowing through said second point;

providing by said third light detection means an output which is effectively indicative of a third dimension of said biomedical agent flowing through said third point;

processing the outputs from said first light detection means, from said second light detection means and from said third light detection means providing said multi-dimension measuring of said biomedical agent and said analysis of said biomedical agent, wherein said analysis includes at least a counting of the biomedical agents, defining a concentration of the biomedical agents, a radioactive absorption testing, a X-Ray absorption testing, an infrared testing, an ultraviolet testing, a magnetic testing, an electromagnetic testing, an electromagnetic radiation absorption testing, a mass spectroscopy testing, a liquid chromatography testing, a flame ionization testing, a chemical testing, a DNA melting point testing, a DNA titration testing, an electrochemical testing, a fluorescent chemical testing, a fluorescence quenching testing, a redox-labeled nucleic acid binding testing, an oscillatory spectral detection testing.

2. The method of claim 1, wherein further said first light beam source provides a first scanning light beam which provides a scanning of each biomedical agent flowing through a first point within said biomedical agent monitoring region, said second light beam source provides a second scanning light beam which provides said scanning of said each biomedical agent flowing through a second point within said biomedical agent monitoring region and said third light beam source provides a third scanning light beam which provides said scanning of said each biomedical agent flowing through said third point located within said biomedical agent monitoring region, and wherein those three scanning light beams provide said scanning appropriately in the directions of said XYZ axes.