Biosensor

• US 8,951,395 B2
• Filed: 11/05/2008
• Issued: 02/10/2015
• Est. Priority Date: 11/05/2007
• Status: Active Grant

• Current Assignee: Nippon Kayaku Company Limited
• Original Assignee: Nippon Kayaku Company Limited
• Inventors: Machida, Reiko, Yokoyama, Takao, Irie, Yayoi, Takagi, Hisako, Umegae, Yoshihiko, Tanabe, Toshio
• Primary Examiner(s): LEONG, SUSAN DANG
• Application Number:
  US12/741,242
• Publication Number:
  US 20110031118A1
• Time in Patent Office: 2,288 Days
• Field of Search: 20440301-40315, 204/411, 204/412, 205/777.5, 205/778, 205/792, 435 4- 4052, 422 681- 98, 436 62- 71, 436500-548
• US Class Current: 204/403.01
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A biosensor for electrochemically measuring a sample may include a first member made of an insulating material, an electrode system including a working electrode and a counter electrode formed on the first member, a second member fixed over the first member, a sample flow channel provided between the first member and the second member, a hydrophilic section provided on at least a part of the internal surface of the sample flow channel and extending from a first end near the electrode system to a second end on the opposite side, and a flow stop area provided on a section adjacent to the second end on the first member or on the internal surface of the sample flow channel.

Claims

1. A biosensor for electrochemically measuring a sample, provided with:

a first member made of an insulating material;

an electrode system including a working electrode and a counter electrode formed on the first member;

a second member fixed over the first member so as to cover at least a part of the electrode system;

a sample flow channel provided between the first member and the second member to allow communication between the outside and a part of the electrode system covered by the second member;

a first end located at a downstream side end of the sample flow channel;

a second end located at an upstream side end of the sample flow channel;

a first hydrophilic section provided on at least a part which is between the first end and the second end contacting with the second end;

a flow stop area provided on a section adjacent to the second end on the first member or on the internal surface of the sample flow channel between the first end and the second end to temporarily stop the flow of the sample into the electrode system;

a sample retaining area which is provided to the first member on an upstream side from the second end, and is for retaining the sample which has been dripped down;

an introduced piece which is provided extending toward the upstream side from the second end side of the sample flow channel of the second member; and

a second hydrophilic section which is provided on the surface of the introduced piece which faces to the sample retaining area, and which communicates with the first hydrophilic section,

wherein a part of the electrode system is exposed to an inside of the sample flow channel at a downstream side of the first end,

wherein the flow stop area is formed of a linear or belt-shaped hydrophobic section which is provided at a downstream side of the sample retaining area so as to stop the sample in a state where it is not contacting the sample flow channel,

wherein the introduced piece is formed of a material having elasticity, which is capable of moving between a first position at which the introduced piece is bent so as to be distal from the first member, and a second position at which the introduced piece is in proximity of the first member from the first position, and

wherein the second position is a position at which the sample is introduced into the sample flow channel by a capillary action which occurs between the introduced piece and the first member.

2. A biosensor according to claim 1, wherein said flow stop area is a linear or belt-shaped hydrophobic section which is provided to the internal surface of said sample flow channel so as to transect said first hydrophilic section in the width direction of said sample flow channel.

3. A biosensor according to claim 1, wherein the sample retaining area is formed to have a hydrophilic surface.

4. A biosensor according to claim 1, wherein the sample retaining area is formed to have a step on the first member which is higher than the other area of the first member.

5. A biosensor according to claim 2, wherein said hydrophobic section has a convex section in which the convexity is directed toward said electrode system.

6. A biosensor according to claim 1, wherein the portion of said first member that is opposite said introduced piece is designed to have the same shape as said introduced piece, with said sample flowing up to said flow stop area once said sample is brought into contact with said introduced piece.

7. A biosensor according to claim 1, wherein a plurality of said electrode systems is provided on said first member, with said sample flow channels provided for these respective said electrode systems.

8. A biosensor according to claim 1, wherein said second member is fixed in place to said first member via a spacer which is provided between said first member and said second member, and the side wall of said sample flow channel is formed from said spacer.

9. A biosensor according to claim 1, wherein a pre-treatment reagent is disposed to at least one of either an area of said sample flow channel that is farther toward said second end than said flow stop area, or an area on said first member which is not covered by said second member, said pre-treatment reagent being employed in a pre-treatment reaction on said sample in order to remove, capture or convert to an inert form interfering substances which would hinder said electrochemical measurement.

10. A biosensor according to claim 1, wherein an oxidoreductase and redox mediator is disposed to at least said working electrode in said electrode system.

11. A biosensor according to claim 10, wherein said redox mediator includes at least one of ruthenium derivative, osmium derivative, ferrocyanide derivative, ferrocene derivative, quinone derivative, phenothiazine derivative, phenoxazine derivative, phenazine derivative, indophenol derivative, diphenylamine derivative or phenol derivative.

12. A biosensor according to claim 10, wherein said oxidoreductase includes at least one of pyranose oxidase, L-sorbose oxidase, 1,5-anhydroglucitoldehydrogenase or L-sorbose dehydrogenase.

13. A biosensor according to claim 9, wherein said pre-treatment reagent includes reagent for removing, capturing or converting glucose to another substance which does not effect said electrochemical measurement.