Matrices and media for storage and stabilization of biomolecules

• US 8,951,719 B2
• Filed: 08/20/2012
• Issued: 02/10/2015
• Est. Priority Date: 09/12/2008
• Status: Active Grant

• Current Assignee: GenTegra, LLC (Genisyss, LLC)
• Original Assignee: GenTegra, LLC (Genisyss, LLC)
• Inventors: Hogan, Michael, Saghbini, Michael, Wong, David, Shi, Chunnian
• Primary Examiner(s): Horlick, Kenneth R.
• Assistant Examiner(s): THOMAS, DAVID C
• Application Number:
  US13/590,025
• Publication Number:
  US 20120308987A1
• Time in Patent Office: 904 Days
• Field of Search: None
• US Class Current: 435/2
• CPC Class Codes: C07H 21/02 with ribosyl as saccharide radical
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C09K 15/30 containing heterocyclic ring with at least one nitrogen atom as ring member
• CPC Class Codes: C12N 15/10 Processes for the isolation, preparation or purification of DNA or RNA chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34
• CPC Class Codes: C12N 5/0632 Cells of the oral mucosa
• CPC Class Codes: C12N 5/0634 Cells from the blood or the immune system
• CPC Class Codes: C12N 9/96 Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: Y10T 428/139 Open-ended, self-supporting conduit, cylinder, or tube-type article
• CPC Class Codes: Y10T 428/1397 Single layer [continuous layer]

Abstract

The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention.

Claims

1. A composition comprising:

boric acid or a salt thereof;

a plasticizer, wherein the plasticizer is a poly-alcohol;

a hydroxyl radical scavenger; and

an RNase inhibitor, wherein the RNase inhibitor is aluminon,

wherein the composition is;

a solid-state or dry-state matrix in a solid or dry form which can be converted to a liquid form by suspending, re-hydrating, or solubilizing the matrix in water;

or

an aqueous medium in a liquid state.

2. The composition of claim 1, further comprising a metal chelater.

3. The composition of claim 2 wherein the metal chelater is EDTA.

4. The composition of claim 1 wherein the hydroxyl radical scavenger is histidine.