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Droplet-based surface modification and washing

  • US 8,951,721 B2
  • Filed: 11/10/2009
  • Issued: 02/10/2015
  • Est. Priority Date: 04/18/2006
  • Status: Active Grant
First Claim
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1. A method of splitting a droplet, the method comprising:

  • (a) providing a droplet microactuator comprising a droplet comprising beads, wherein the droplet actuator comprises;

    (i) a first substrate comprising electrodes configured for manipulating a droplet on a surface of the substrate; and

    (ii) a second substrate arranged relative to the first substrate and spaced from the surface of the first substrate by a distance sufficient to define a space between the first substrate and second substrate, wherein the droplet is situated in the space between the first substrate and second substrate;

    (b) immobilizing the beads; and

    (c) conducting one or more droplet operations to divide the droplet to yield a set of droplets comprising;

    (i) a droplet comprising the beads; and

    (ii) a droplet substantially lacking the beads.

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