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Compositions for detecting small RNAs

  • US 8,951,730 B2
  • Filed: 03/07/2013
  • Issued: 02/10/2015
  • Est. Priority Date: 11/25/2008
  • Status: Expired due to Fees
First Claim
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1. An amplification reaction mixture comprising:

  • (a) a miR-221 target capture probe that forms under hybridizing conditions at least a partially double-stranded structure that is a hairpin structure made up of a contiguous linear sequence that includes an internal target hybridizing sequence that is substantially complementary to all or a portion of SEQ ID NO;

    44 and that forms a single-stranded loop portion of the hairpin structure, wherein the internal target hybridizing sequence is flanked by a capture region and by a terminal region that binds to the capture region to form a double-stranded stem portion of the hairpin structure, wherein the capture region further comprises a substantially homopolymeric nucleic acid sequence and the terminal region further comprises a substantially homopolymeric nucleic acid sequence that is complementary to the capture region sequence, and wherein the miR-221 target capture probe is at least 95% identical to SEQ ID NO;

    24; and

    (b) a first amplification oligomer configured to hybridize to a miR-221 target nucleic acid that is an extender primer comprising a 3′

    target hybridizing sequence and a 5′

    extension nucleic acid sequence, wherein the 3′

    target hybridizing sequence is substantially identical to about 9 or more contiguous nucleic acids at the 5′

    end of the target hybridizing sequence of the miR-221 target capture probe.

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