Plant promoter operable in basal endosperm transfer layer of endosperm and uses thereof
First Claim
1. An expression construct comprising a promoter operably connected to a heterologous transgene, wherein said promoter confers expression of said transgene in the basal endosperm transfer layer (BETL) of an endosperm and comprises a nucleotide sequence selected from the group consisting of:
- (i) the nucleotide sequence of SEQ ID NO;
2; and
(ii) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of (i).
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Abstract
The present invention provides an isolated promoter or an active fragment or derivative thereof capable of conferring selective expression on a gene to which it is operably connected in the endosperm of a developing plant seed and preferably in the basal endosperm transfer layer (BETL) of endosperm. The present invention also provides expression vectors and constructs and transgenic plant cells, plant parts and whole plants comprising the promoter, active fragments and derivatives, and well as methods of modulating one or more plant phenotypes employing the promoter, active fragments and derivatives.
9 Citations
15 Claims
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1. An expression construct comprising a promoter operably connected to a heterologous transgene, wherein said promoter confers expression of said transgene in the basal endosperm transfer layer (BETL) of an endosperm and comprises a nucleotide sequence selected from the group consisting of:
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(i) the nucleotide sequence of SEQ ID NO;
2; and(ii) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of (i). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for expressing a nucleic acid in developing endosperm, basal endosperm, or BETL cells, comprising:
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(i) obtaining a transgenic plant, transgenic plant cell or transgenic plant part comprising an expression construct comprising a promoter operably connected to a nucleic acid; and (ii) maintaining said transgenic plant, transgenic plant cell or transgenic plant part for a time and under conditions sufficient for said nucleic acid to be expressed, wherein said promoter comprises a nucleotide sequence selected from the group consisting of; (i) the nucleotide sequence of SEQ ID NO;
2; and(ii) a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of (i); and wherein said promoter confers expression of said nucleic acid in developing endosperm, basal endosperm, or BETL cells. - View Dependent Claims (12, 13, 14, 15)
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Specification