Micro fluidic optic design

• US 8,961,764 B2
• Filed: 10/14/2011
• Issued: 02/24/2015
• Est. Priority Date: 10/15/2010
• Status: Active Grant

• Current Assignee: Leidos Innovations Technology, Inc.
• Original Assignee: Lockheed Martin Corporation (Martin Marietta Corporation), Zygem Corporation Ltd.
• Inventors: Root, Brian E., Scott, Orion N., Landers, James P., Trost, Peter Karl, Egan, Michael E., South, Doug
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US13/273,947
• Publication Number:
  US 20120090996A1
• Time in Patent Office: 1,229 Days
• US Class Current: 204/452
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01J 3/18 using diffraction elements, e.g. grating gratings per se G02B
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/6484 Optical fibres
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

A DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first separation channel for electrophoretic separation of DNA fragments in a first sample. Further, the DNA analyzer includes a first optical device. The first optical device includes an illuminating path and a detecting path. The illuminating path directs a first input light beam received from a light source to a first separation channel of the microfluidic chip. The first input light beam causes fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light. The detecting path collects and directs the first fluorescent light to a first plurality of optical fibers. Further, the DNA analyzer includes a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light.

Claims

1. An apparatus, comprising:

a first optical device comprising;

an illuminating path that directs a first input light beam received from a light source to a first separation channel of a microfluidic chip, the first input light beam causing fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light, the illuminating path being configured to receive the first input light beam from the light source via an input optical fiber; and

a detecting path that collects and directs the first fluorescent light to a first plurality of optical fibers;

a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light; and

an optical fiber connector configured to connect the first input optical fiber and the first plurality of output fibers with the first optical device, the optical fiber connector being configured to connect the first input optical fiber at a center position, and the first plurality of output fibers around the center position.

2. The apparatus of claim 1, wherein the first optical device further comprises:

a first set of optic elements; and

a first motion control module configured to align the first set of optic elements to the first separation channel.

3. The apparatus of claim 2, wherein the first motion control module is configured to align the first set of optic elements based on detection output of the spectrometer.

4. The apparatus of claim 1, wherein the first optical device further comprises:

an objective lens configured to focus the first input light beam to the first separation channel based on detection output of the spectrometer.

5. The apparatus of claim 1, wherein the first optical device comprises:

a filter configured to filter out fluorescence in the first input light beam.

6. The apparatus of claim 1, wherein the spectrometer further comprises:

a dispersive element configured to spatially separate the fluorescent components; and

an array of photo detection units configured to detect the spatially separated fluorescent components.

7. The apparatus of claim 6, wherein the array of photo detection units is within a charge-coupled device (CCD) system.

8. The apparatus of claim 1, further comprising:

a second optical device comprising;

an illuminating path that directs a second input light beam received from the light source to a second separation channel of the microfluidic chip, the second input light beam causing fluorescent labels attached on DNA fragments in the second separation channel to emit a second fluorescent light; and

a detecting path that collects and directs the second fluorescent light to a second plurality of optical fibers.

9. The apparatus of claim 8, wherein the spectrometer comprises:

another optical fiber connector configured to connect the first plurality of output optical fibers and the second plurality of output optical fibers with the spectrometer.

10. The apparatus of claim 9, wherein

the other optical fiber connector is configured to stack the first plurality of output optical fibers and the second plurality of output optical fibers in a line.

11. A method, comprising:

transmitting, by a first input optical fiber at a center position of an optical fiber connector, the first input light beam from a light source to an illuminating path;

directing, by the illuminating path, a first input light beam to a first separation channel of a microfluidic chip, the first input light beam causing fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescent light;

collecting the first fluorescent light;

transmitting, by a first plurality of output optical fibers around the center position of the optical fiber connector, the first fluorescent light to a spectrometer; and

detecting, by the spectrometer, fluorescent components in the first fluorescent light.

12. The method of claim 11, further comprising:

aligning a first set of optic elements to the first separation channel based on detection output of the spectrometer.

13. The method of claim 11, further comprising:

filtering out fluorescence in the first input light beam.

14. The method of claim 11, wherein detecting, by the spectrometer, the fluorescent components in the first output light beam further comprises:

spatially separating the fluorescent components;

detecting, by an array of photo detection units, the spatially separated fluorescent components.

15. A DNA analyzer, comprising:

an interface for coupling a microfluidic chip to the DNA analyzer, wherein the microfluidic chip includes a first separation channel for electrophoretic separation of DNA fragments in a first sample;

a first optical device comprising;

an illuminating path that directs a first input light beam received from a light source to the first separation channel of the microfluidic chip, the first input light beam causing fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light, the illuminating path being configured to receive the first input light beam from the light source via a first input optical fiber; and

a detecting path that collects and directs the first fluorescent light to a first plurality of optical fibers;

a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light; and

an optical fiber connector configured to connect the first input optical fiber and the first plurality of output fibers with the first optical device, the optical fiber connector being configured to connect the first input optical fiber at a center position, and the first plurality of output fibers around the center position.

16. The DNA analyzer of claim 15, wherein the first optical device further comprises:

a first set of optic elements; and

a first motion control module configured to adjust the first set of optic elements to align the first set of optic elements to the first separation channel.

17. The DNA analyzer of claim 16, wherein the first motion control module is configured to adjust the first set of optic elements based on detection output of the spectrometer.

18. The DNA analyzer of claim 15, wherein the first optical device further comprises:

an objective lens configured to focus the first input light beam to the first separation channel based on detection output of the spectrometer.

19. The DNA analyzer of claim 15, wherein the first optical device comprises:

a filter configured to filter out fluorescence in the first input light beam.

20. The DNA analyzer of claim 15, wherein the spectrometer further comprises:

a dispersive element configured to spatially separate the fluorescent components; and

an array of photo detection units configured to detect the spatially separated fluorescent components.

21. The DNA analyzer of claim 20, wherein the array of photo detection units is within a charge coupled device (CCD) system.

22. The DNA analyzer of claim 15, further comprising:

a second optical device comprising;

an illuminating path that directs a second input light beam received from the light source to a second separation channel of the microfluidic chip, the second input light beam causing fluorescent labels attached on DNA fragments in the second separation channel to emit a second fluorescence light; and

a detecting path that collects and directs the second fluorescent light to a second plurality of optical fibers.

23. The DNA analyzer of claim 22, wherein the spectrometer comprises:

another optical fiber connector configured to connect the first plurality of output optical fibers and the second plurality of output optical fibers with the spectrometer.

24. The DNA analyzer of claim 23, wherein

the other optical fiber connector is configured to stack the first plurality of output optical fibers and the second plurality of output optical fibers in a line.