Compositions and methods for production of immunoglobulins

• US 8,962,278 B2
• Filed: 08/03/2006
• Issued: 02/24/2015
• Est. Priority Date: 08/03/2005
• Status: Active Grant

• Current Assignee: iBio, Inc.
• Original Assignee: iBio, Inc.
• Inventors: Yusibov, Vidadi, Mett, Vadim, Hull, Anna
• Primary Examiner(s): Zeman, Robert A
• Application Number:
  US11/498,522
• Publication Number:
  US 20100239594A1
• Time in Patent Office: 3,127 Days
• Field of Search: None
• US Class Current: 435/91.2
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/10 Antimycotics
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 33/10 Anthelmintics
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 35/02 specific for leukemia
• CPC Class Codes: A61P 37/06 Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07K 16/1278 from Bacillus (G)
• CPC Class Codes: C07K 2317/13 isolated from plants
• CPC Class Codes: C07K 2317/21 from primates, e.g. man
• CPC Class Codes: C07K 2317/565 Complementarity determining region [CDR]

Abstract

Provided are oligonucleotides for isolating human antibody cDNAs from cells or cell lines, such as hybridomas. The invention also provides cDNAs that encode at least one provided CDR of a heavy chain or a light chain of a human monoclonal antibody that binds to B. anthracis protective antigen; and cDNAs that encode at least one provided CDR of a heavy chain or a light chain of a human monoclonal antibody that binds to B. anthracis lethal factor. The invention further provides expression vectors that contain one or more cDNAs isolated according to the methods of the invention, host cells expressing one or more inventive cDNAs, and transgenic plants and animals that express one or more inventive cDNAs. In certain embodiments of the invention the expression system is a plant-based expression system. The invention further provides antibody compositions comprising one or more antibodies produced by expressing a cDNA isolated according to the methods of the invention in a suitable expression system. Additionally encompassed in the invention are kits containing one or more of provided compositions, as well as methods of production and use of provided compositions.

Claims

1. A method of isolating a nucleic acid that encodes an antibody heavy chain comprising the sequence set forth in SEQ ID NO:

48 or SEQ ID NO;

52, comprising amplifying said nucleic acid from a cell or cell line that produces the antibody heavy chain using a primer mix of comprising;

SEQ ID NOS;

2, 13, 14, and 15,

SEQ ID NOS;

2, 16, 17, and 18,

SEQ ID NOS;

1, 7, 8, and 9, or

SEQ ID NOS;

1, 10, 11, and 12.

2. The method of claim 1, comprising using a primer comprising the sequence set forth in SEQ ID NO:

2 in combination with primers comprising the sequences set forth in SEQ ID NOS;

13, 14, and 15, or SEQ ID NOS;

16, 17, and 18.

3. The method of claim 1, comprising using a primer comprising the sequence set forth in SEQ ID NO:

1 in combination with primers comprising the sequences set forth in SEQ ID NOS;

7, 8, and 9, or SEQ ID NOS;

10, 11, and 12.

4. The method of claim 1, comprising using a primer consisting of the sequence set forth in SEQ ID NO:

2 in combination with primers consisting of the sequences set forth in SEQ ID NOS;

13, 14, and 15, or SEQ ID NOS;

16, 17, and 18.

5. The method of claim 1, comprising using a primer consisting of the sequence set forth in SEQ ID NO:

1 in combination with primers consisting of the sequences set forth in SEQ ID NOS;

7, 8, and 9, or SEQ ID NOS;

10, 11, and 12.