Enhanced animal cell growth using ultrasound

• US 8,962,290 B2
• Filed: 09/21/2011
• Issued: 02/24/2015
• Est. Priority Date: 08/26/2008
• Status: Active Grant

• Current Assignee: Hidaca Limited
• Original Assignee: IntelligentNano Inc.
• Inventors: Chen, Jie, Xing, James, Ang, Woon T., Gul, Hilal
• Primary Examiner(s): Ford, Allison
• Assistant Examiner(s): Pyla, Yvonne
• Application Number:
  US13/238,947
• Publication Number:
  US 20130022957A1
• Time in Patent Office: 1,252 Days
• Field of Search: None
• US Class Current: 435/173.8
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/2812 against CD4
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: C12N 2511/00 Cells for large scale production
• CPC Class Codes: C12N 2521/10 Sound, e.g. ultrasounds
• CPC Class Codes: C12N 5/0647 Haematopoietic stem cells; Uncommitted or multipotent progenitors
• CPC Class Codes: C12N 5/163 one of the fusion partners being a B or a T lymphocyte

Abstract

A method of increasing animal cell growth and monoclonal antibody production in an animal cell or cell culture includes the use of ultrasound at a frequency greater than about 1 MHz.

Claims

1. A method of increasing the rate of growth of an animal cell culture, comprising exposing the animal cell culture to ultrasound having a frequency from greater than about 1 MHz to 2 MHz,

wherein the ultrasound is pulsed, and

the animal cell culture is a hybridoma cell culture or a CHO cell culture.

2. The method of claim 1, wherein the frequency of the ultrasound is between about 1.4 MHz and about 1.6 MHz.

3. The method of claim 2 wherein the frequency of the ultrasound is about 1.5 MHz.

4. The method of claim 1, wherein the intensity of the ultrasound is from about 1 mW/cm² to about 5 W/cm².

5. The method of claim 4, wherein the intensity of the ultrasound is from about 10 mW/cm² to about 150 mW/cm2.

6. The method of claim 1, wherein the ultrasound is applied in periodic intervals.

7. The method of claim 6, wherein the ultrasound is applied in one or more intervals totalling less than about 60 minutes in a 24 hour period.

8. The method of claim 6, wherein the ultrasound is applied in a single interval of about 10 to about 20 minutes in a 24 hour period.

9. The method of claim 1, wherein the animal cell culture is a hybridoma cell culture.

10. The method of claim 9, wherein the hybridoma cell culture is capable of producing a monoclonal antibody.

11. The method of claim 10, wherein the monoclonal antibody comprises an anti-CD4 monoclonal antibody.

12. A method of enhancing monoclonal antibody production from a hybridoma cell culture or a CHO cell culture, comprising the steps of subjecting the hybridoma cell culture or CHO cell culture to ultrasound in accordance with claim 1.

13. A method of producing antibodies, comprising exposing an animal cell culture of antibody producing animal cells to ultrasound having a frequency from greater than about 1 MHz to 2 MHz, wherein the ultrasound is pulsed.

14. The method of claim 13, wherein the animal cells are hybridoma cells or CHO cells.

15. The method of claim 13, wherein the frequency of the ultrasound is between about 1.4 MHz and about 1.6 MHz.

16. The method of claim 13, wherein the animal cells comprise recombinant DNA.

17. The method of claim 13 wherein the frequency of the ultrasound is about 1.5 MHz.

18. The method of claim 13, wherein the ultrasound is applied in periodic intervals.