Method for repairing DNA damage in keratinocytes

• US 8,962,571 B2
• Filed: 04/20/2012
• Issued: 02/24/2015
• Est. Priority Date: 02/09/2009
• Status: Active Grant

• Current Assignee: ELC Management LLC (The Estee Lauder Companies Inc.)
• Original Assignee: ELC Management LLC (The Estee Lauder Companies Inc.)
• Inventors: Maes, Daniel H., Pernodet, Nadine A., Slutsky, Lenny, Collins, Donald F., Goldgraben, Kerri, Pelle, Edward, McCarthy, James Timothy
• Primary Examiner(s): Lukton, David
• Application Number:
  US13/452,415
• Publication Number:
  US 20120219537A1
• Time in Patent Office: 1,040 Days
• Field of Search: 514/21.9, 514/21.5, 514/21.6, 514/21.7, 514/21.8, 514/18.6
• US Class Current: 514/21.9
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/06 Tripeptides
• CPC Class Codes: A61K 38/08 Peptides having 5 to 11 amino acids A61K38/043 - A61K38/046 take precedence
• CPC Class Codes: A61K 38/10 Peptides having 12 to 20 amino acids A61K38/043 - A61K38/046 take precedence
• CPC Class Codes: A61K 8/645 Proteins of vegetable origin; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/66 Enzymes
• CPC Class Codes: A61K 8/9789 Magnoliopsida [dicotyledons]
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin

Abstract

A method for repairing DNA damage in human keratinocytes by applying to the keratinocytes a composition comprising at least one CLOCK or PER1 gene activator and at least one DNA repair enzyme.

Claims

1. A method for inhibiting UV damage in keratinocytes by applying to the skin of a human subject in need thereof a cosmetic composition comprising at least one CLOCK or PER1 gene activator and inactivated bacterial lysates from Bifido bacterium.

2. The method of claim 1 wherein the CLOCK or PER1 gene activator is a peptide having from about 3 to 13 amino acid residues and of the formula (I) (SEQ ID NO:

1);

R₁-(AA)_n-X₁-Ser-Thr-Pro-X₂-(AA)_p-R₂

wherein;

X₁ represents threonine, serine, or is a covalent bond,

X₂ represents an isoleucine, leucine, proline, valine, alanine, glycine, or is a covalent bond,

AA represents any amino acid or derivative thereof, and n and p are whole numbers between 0 and 4,

R₁ is bonded to the N-terminal amino acid of the peptide and is a hydrogen atom or a substituent selected from benzoyl group, a tosyl group, or a benzyloxycarbonyl,

R₂ is bonded to the carboxyl group of the C-terminal amino acid of formula I, and is selected from the group consisting of hydroxyl, a C₁ to C₂₀ alkoxy, NH₂, NHY, or N(Y)Y group with Y a C₁ to C₄ alkyl chain.

3. The method of claim 1 wherein the CLOCK or PER1 gene activators are selected from the group consisting of:

4. The method of claim 3 wherein the CLOCK or PER1 gene activators are selected from:

5. The method of claim 2 wherein the CLOCK or PER1 gene activator is a peptide having the formula Ser-Thr-Pro-NH₂.

6. The method of claim 2 wherein the CLOCK or PER1 gene activator is a peptide having the formula:

Ser-Thr-Pro-NH₂ and the DNA repair enzyme is selected from the group consisting of;

base excision repair (BER) enzymes,

nucleotide excision repair (NER) enzymes,

DNA polymerases,

DNA helicases,

Mismatch repair (MMR) enzymes;

and mixtures thereof.

7. The method of claim 2 wherein the CLOCK or PER1 gene activator is a peptide having the formula Ser-Thr-Pro-NH₂ and the inactivated bacterial lysate from Bifido bacterium is Bifida ferment lysate.

8. The method of claim 1 wherein the composition further comprises at least one additional botanical extract.

9. The method of claim 1 wherein the composition further comprises at least one free radical scavenger operable to inactivate singlet oxygen in skin keratinocytes.

10. The method of claim 1 wherein the composition further comprises at least one aqueous phase structuring agent comprising a polysaccharide, an acrylic polymer, or mixtures thereof.

11. The method of claim 1 wherein the composition further comprises at least one vitamin or vitamin derivative.

12. The method of claim 1 wherein the cosmetic composition is in the form of a solution, dispersion, suspension, or emulsion.

13. The method of claim 1 wherein the composition is applied to the skin prior to nightly rest.

14. The method of claim 1 wherein the composition is applied to the skin as part of a regimen comprised of cleansing, toning, and application of the composition to the skin.

15. The method of claim 1 wherein the keratinocytes are facial keratinocytes.

16. The method of claim 1 wherein the composition is applied to the skin twice per day.

17. A method for inhibiting UV damage in skin comprising applying to the skin of a human subject in need thereof a composition comprising at least one CLOCK or PER1 gene activator and a DNA repair enzyme contained in one or more of the following:

Arabidopsis Thaliana extract either alone or in admixture with lecithin and water;

Lactobacillus ferment,

Micrococcus lysate,

Plankton extract,

Bifida ferment lysate; and

mixtures thereof.

18. The method of claim 17 wherein the DNA repair enzyme is contained in inactivated bacterial lysates of Bifido bacterium.

19. The method of claim 17 wherein the DNA repair enzyme is contained in Micrococcus lysate.

20. The method of claim 17 wherein the DNA repair enzyme is contained in Lactobacillus ferment.