Method of pre-treatment and staining of a biological sample and device for support of a biological sample and methods of using such device

• US 8,969,087 B2
• Filed: 09/06/2012
• Issued: 03/03/2015
• Est. Priority Date: 06/29/2004
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Dako Denmark A/S (Agilent Technologies, Inc.)
• Inventors: Bland, Loren L., Winther, Lars, Stanforth, David A., Lindberg, Martin N., Key, Marc E.
• Primary Examiner(s): SIEFKE, SAMUEL P
• Application Number:
  US13/605,228
• Publication Number:
  US 20120330564A1
• Time in Patent Office: 908 Days
• Field of Search: 422/500, 422/502, 422/50, 422/68.1, 422/63, 436/63
• US Class Current: 436/63
• CPC Class Codes: B01L 2300/023 Sending and receiving of information, e.g. using bluetooth
• CPC Class Codes: B01L 2300/024 Storing results with means integrated into the container
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 3/545 for laboratory containers
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00782 reprogrammmable code
• CPC Class Codes: G01N 35/00732 Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

There is disclosed a method of pre-treatment and staining, according to a protocol, of a biological sample disposed upon the surface of a carrier, the method comprising the step of recording at least one parameter relating to at least one protocol step in a non-volatile memory located either upon or within the carrier or a device incorporating the carrier. Also disclosed is a device comprising: a non-volatile memory; a surface of the device adapted to carry a biological sample; and communications means electrically coupled to the memory for enabling data transmission to or from an external apparatus. Also disclosed is a method of controlling processing of a biological sample disposed upon a carrier, comprising: providing, upon or within the carrier or an apparatus holding the carrier, a non-volatile memory having information relating to sample processing priority or protocol; reading the information; and scheduling the processing based upon the information.

Claims

1. An apparatus for processing a biological sample comprising:

at least one station configured to receive at least one sample carrier; and

at least one instrument comprising;

a stainer that dispenses a reagent with a robotic arm;

a radio frequency transmitter configured to transmit a radio frequency signal to a radio frequency device associated with a sample carrier, the sample carrier having at least one biological sample arranged thereon;

a radio frequency receiver configured to receive data stored on the radio frequency device via a return signal from the radio frequency device; and

a computer communication interface comprising a non-transitory memory programmed with instructions that cause the computer communication interface to

convey the data to a central database in a computer; and

receive control instructions from the computer to carry out at least one processing step on the biological sample,

wherein the computer is configured to determine the at least one processing step to be carried out on the biological sample based on the data.

2. The apparatus of claim 1, wherein the data stored on the radio frequency device includes at least one of patient data, carrier priority data, access data, historical processing data for the sample, sample type data, or the position of the sample on the carrier.

3. The apparatus of claim 1, wherein the radio frequency device includes writable memory.

4. The apparatus of claim 3, wherein the laboratory instrument further comprises a writing facility configured to write data received from the computer to the writable memory of the radio frequency device.

5. The apparatus of claim 1, wherein the data further includes information about at least one processing step performed on the sample.

6. The apparatus of claim 1, wherein the computer is further configured communicate with the radio frequency device in order to determine a next processing step.

7. The apparatus of claim 6, wherein the sample carrier includes writable memory, and the laboratory instrument comprises a writing facility configured to write information regarding the processing to the writable memory.

8. The apparatus of claim 1, wherein the radio frequency device is embedded in the sample carrier.

9. The apparatus of claim 1, wherein at least one of the computer and the laboratory instrument comprises a database communication interface configured to transfer to a remote database data relating to the processing performed by the at least one instrument.

10. The apparatus of claim 1, wherein the radio frequency device includes an RF antenna.

11. The apparatus of claim 1, wherein the radio frequency device includes a data processing unit with a microprocessor.

12. An apparatus for processing a biological tissue specimen comprising:

at least one station configured to receive at least one sample carrier; and

at least one instrument comprising;

a stainer that dispenses a reagent with a robotic arm;

a radio frequency transmitter configured to transmit a radio frequency signal to a radio frequency device associated with a sample carrier, the sample carrier having at least one biological tissue specimen arranged thereon;

a radio frequency receive configured to receive data stored on the radio frequency device via a return signal from the radio frequency device; and

a computer communication interface comprising a non-transitory memory programmed with instructions that cause the computer communication interface to

convey the data to a central database in a computer; and

receive control instructions from the computer to carry out at least one processing step on the biological tissue specimen,

wherein the computer is configured to determine the at least one processing step to be carried out on the biological tissue specimen based on the data.

13. An apparatus for processing a biological sample comprising:

at least one stainer that dispenses a reagent with a robotic arm, comprising;

at least one station configured to receive at least one sample carrier;

a radio frequency transmitter configured to transmit a radio frequency signal to a radio frequency device associated with a sample carrier, the sample carrier having at least one sample arranged thereon;

a radio frequency receiver configured to receive data stored on the radio frequency device via a return signal from the radio frequency device; and

a computer communication interface comprising a non-transitory memory programmed with instructions that cause the computer communication interface to

convey the data to a central database in a computer; and

receive control instructions from the computer to carry out at least one processing step on the sample,

wherein the computer is configured to determine the at least one processing step to be carried out on the sample based on the data.