Serum albumin binding molecules
First Claim
Patent Images
1. A polypeptide comprising a fibronectin type III tenth (10Fn3) domain, wherein the 10Fn3 domain binds to human serum albumin with a KD of 1 μ
- M or less, wherein the 10Fn3 domain comprises a modified amino acid sequence in one or more of the BC, DE and FG loops relative to the wild-type human 10Fn3 domain (SEQ ID NO;
1), and wherein the polypeptide comprises an amino acid sequence at least 75% identical to an amino acid sequence selected from SEQ ID NOs;
4, 8, 12, 16, 20, and 24-44.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to an antibody-like protein based on the tenth fibronectin type III domain (10Fn3) that binds to serum albumin. The invention further relates to fusion molecules comprising a serum albumin-binding 10Fn3 joined to a heterologous protein for use in diagnostic and therapeutic applications.
-
Citations
33 Claims
-
1. A polypeptide comprising a fibronectin type III tenth (10Fn3) domain, wherein the 10Fn3 domain binds to human serum albumin with a KD of 1 μ
- M or less, wherein the 10Fn3 domain comprises a modified amino acid sequence in one or more of the BC, DE and FG loops relative to the wild-type human 10Fn3 domain (SEQ ID NO;
1), and wherein the polypeptide comprises an amino acid sequence at least 75% identical to an amino acid sequence selected from SEQ ID NOs;
4, 8, 12, 16, 20, and 24-44. - View Dependent Claims (2, 3, 4, 5, 6, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 33)
- M or less, wherein the 10Fn3 domain comprises a modified amino acid sequence in one or more of the BC, DE and FG loops relative to the wild-type human 10Fn3 domain (SEQ ID NO;
-
7. A fusion polypeptide comprising a fibronectin type III tenth (10Fn3) domain and a heterologous protein, wherein the 10Fn3 domain binds to human serum albumin with a KD of 1 μ
- M or less and comprises an amino acid sequence at least 75% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs;
4, 8, 12, 16, 20, and 24-44. - View Dependent Claims (8, 9, 10, 11, 31, 32)
- M or less and comprises an amino acid sequence at least 75% identical to an amino acid sequence selected from the group consisting of SEQ ID NOs;
-
12. A polypeptide comprising a fibronectin type III tenth (10Fn3) domain, wherein the 10Fn3 domain (i) comprises a modified amino acid sequence in one or more of the AB, BC, CD, DE, EF and FG loops relative to the wild-type 10Fn3 domain, (ii) binds to a target molecule not bound by the wild-type 10Fn3 domain, and (iii) comprises a C-terminal tail having a sequence (ED)n, wherein n is an integer from 3 to 7.
Specification