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Methods for the purification of polypeptide conjugates comprising polyalkylene oxide using hydrophobic interaction chromatography

  • US 8,969,532 B2
  • Filed: 10/03/2007
  • Issued: 03/03/2015
  • Est. Priority Date: 10/03/2006
  • Status: Active Grant
First Claim
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1. A method of making a composition comprising a first polypeptide conjugate, said first polypeptide conjugate comprising a first number of poly(alkylene oxide) moieties covalently linked to said first polypeptide, wherein at least one of said poly(alkylene oxide) moieties is covalently linked to said first polypeptide via a glycosyl linking group, wherein said glycosyl linking group is either covalently linked to an amino acid residue of said first polypeptide or is covalently linked to a glycosyl moiety of said first polypeptide, said method comprising:

  • (a) contacting a mixture comprising said first polypeptide conjugate with an anion exchange medium;

    (b) eluting said first polypeptide conjugate from said anion exchange medium, forming a first eluate comprising said first polypeptide conjugate;

    (c) contacting said first eluate with a hydrophobic interaction chromatography (HIC) medium; and

    (d) eluting said first polypeptide conjugate from said hydrophobic interaction chromatography medium,thereby making said composition comprising said first polypeptide conjugate.

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