Algorithms for calibrating an analyte sensor
First Claim
Patent Images
1. A method for determining glucose concentration in a physiologic fluid, the method comprising:
- providing an optical sensor comprising a non-enzymatic, equilibrium fluorescence chemical indicator system disposed along a distal region of an optical fiber, the chemical indicator system comprising a fluorophore operably coupled to a glucose binding moiety, wherein the fluorophore is configured to generate a fluorescent emission signal upon excitation with light, and wherein glucose binding to the glucose binding moiety causes a change in the fluorescent emission signal related to the glucose concentration in the physiologic fluid;
contacting the chemical indicator system with the physiologic fluid;
exciting the fluorophore with light, thereby generating a fluorescent emission signal related to the glucose concentration in the physiologic fluid;
detecting the intensity of the fluorescent emission signal;
obtaining a value of the fluorescent emission signal intensity in the absence of glucose;
obtaining a value of the asymptotic intensity of the fluorescent emission signal at infinite glucose;
obtaining a value of the glucose concentration at which the fluorescent emission intensity is one-half the difference between the fluorescent emission signal intensity in the absence of glucose and the asymptotic intensity of the fluorescent emission signal at infinite glucose; and
processing the fluorescent emission signal intensity by transforming the fluorescent emission signal intensity into a glucose concentration value using the equation;
[G]=c*(I−
a)/(a+b−
I), wherein[G] is the glucose concentration,I is the fluorescent emission signal intensity,a is the value of the fluorescent emission signal intensity in the absence of glucose,b is the value of the asymptotic signal intensity at infinite glucose concentration, minus the fluorescent signal intensity in the absence of glucose (a), andc is the value of the glucose concentration at which the fluorescent signal intensity is one-half the difference between the asymptotic value (b) and the background (a).
2 Assignments
0 Petitions
Accused Products
Abstract
Disclosed are embodiments that relate to algorithms and methods for calibrating an analyte sensor, and more particularly, to algorithms for calibrating an optical glucose sensor comprising an equilibrium fluorescent chemical indicator system. In particular, a method of detecting an analyte concentration is disclosed where a modified Michaelis-Menten equation comprising Michaelis-Menten parameters is used to characterize the signal generated by the analyte sensor.
-
Citations
10 Claims
-
1. A method for determining glucose concentration in a physiologic fluid, the method comprising:
-
providing an optical sensor comprising a non-enzymatic, equilibrium fluorescence chemical indicator system disposed along a distal region of an optical fiber, the chemical indicator system comprising a fluorophore operably coupled to a glucose binding moiety, wherein the fluorophore is configured to generate a fluorescent emission signal upon excitation with light, and wherein glucose binding to the glucose binding moiety causes a change in the fluorescent emission signal related to the glucose concentration in the physiologic fluid; contacting the chemical indicator system with the physiologic fluid; exciting the fluorophore with light, thereby generating a fluorescent emission signal related to the glucose concentration in the physiologic fluid; detecting the intensity of the fluorescent emission signal; obtaining a value of the fluorescent emission signal intensity in the absence of glucose; obtaining a value of the asymptotic intensity of the fluorescent emission signal at infinite glucose; obtaining a value of the glucose concentration at which the fluorescent emission intensity is one-half the difference between the fluorescent emission signal intensity in the absence of glucose and the asymptotic intensity of the fluorescent emission signal at infinite glucose; and processing the fluorescent emission signal intensity by transforming the fluorescent emission signal intensity into a glucose concentration value using the equation;
[G]=c*(I−
a)/(a+b−
I), wherein[G] is the glucose concentration, I is the fluorescent emission signal intensity, a is the value of the fluorescent emission signal intensity in the absence of glucose, b is the value of the asymptotic signal intensity at infinite glucose concentration, minus the fluorescent signal intensity in the absence of glucose (a), and c is the value of the glucose concentration at which the fluorescent signal intensity is one-half the difference between the asymptotic value (b) and the background (a). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
Specification