Illuminator for visualization of fluorophores

• US 8,974,651 B2
• Filed: 04/17/2011
• Issued: 03/10/2015
• Est. Priority Date: 04/17/2010
• Status: Active Grant

• Current Assignee: CC IMEX
• Original Assignee: C.C. Imex
• Inventors: Chan, Richard, Walker, Winston Glenn, Wong, Rita M., In, Jason
• Primary Examiner(s): Van, Luan
• Assistant Examiner(s): Kaur, Gurpreet
• Application Number:
  US13/088,390
• Publication Number:
  US 20110253541A1
• Time in Patent Office: 1,423 Days
• Field of Search: 204/456, 204/450, 204/461, 204/466, 204/600, 204/606, 204/612, 204616-618, 356/344, 356432-444
• US Class Current: 204/461
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

The present invention discloses apparatuses and methods for the visualization of fluorophores in biological systems such as electrophoresis gels and cell cultures. Preferred embodiments of the apparatuses consists of a tray or tank having one or more light sources disposed to direct light through an electrophoresis gel disposed within the vessel such that the luminescence from fluorophores in the gel are easily visualized.

Claims

1. An apparatus useful for the visualization of fluorophores in biological systems comprising:

a vessel configured to contain an electrophoresis gel wherein the electrophoresis gel comprises a top planar surface, a bottom planer surface parallel to the top planar surface and at least first and second planar edges perpendicular to the plane of the top and bottom planar surfaces, the planar edges being adjacent each other in that the first planar edge is contiguous to the second planar edge;

at least a first light source configured to provide orthogonal illumination to the first planar edge of the electrophoresis gel disposed within the gel vessel such that light emitted from one or more fluorophores within the gel is emitted through the top surface or bottom surface of the gel such that the fluorophores can be visualized by a viewer or an optical instrument placed adjacent to the top or bottom surface of the gel; and

at least a second light source configured to provide orthogonal illumination to the second planar edge.

2. The apparatus of claim 1 wherein the vessel is a polygonal shaped vessel comprising a base having flat inner surface and a plurality of vertical walls less than or equal to the number of edges of the polygon, wherein each vertical wall has an inner surface and wherein the vessel is dimensioned to contain the electrophoresis gel;

and wherein said at least one light source is at least one first vertical wall light source attached to the inner surface of a first vertical wall.

3. The apparatus of claim 1 further comprising one or more optical filters disposed between the top planar surface of the gel and the viewer or the optical instrument such that the optical filter is selected to function as an aid for visualization of fluorescence emitted from the gel.

4. The apparatus of claim 1 wherein said at least one light source is a visible light source.

5. The apparatus of claim 1 wherein said at least one light source is an ultraviolet light source.

6. The apparatus of claim 1 wherein said at least one light source comprises one or more light emitting diodes.

7. The apparatus of claim 2 wherein the shape of the polygonal shaped vessel base is similar to the shape of the base of the gel disposed therein.

8. The apparatus of claim 2 wherein the polygonal shaped vessel base is a rectangular vessel base.

9. The apparatus of claim 2 wherein the second light source is attached to the inner surface of a second vertical wall.

10. The apparatus of claim 9 wherein the first vertical wall and second vertical wall are adjacent.

11. The apparatus of claim 9 wherein the first vertical wall and second vertical wall are opposed.

12. The apparatus of claim 9 further comprising at least one third vertical wall light source attached to the inner surface of a third vertical wall wherein the third vertical wall light source is positioned to provide orthogonal illumination to a third vertical edge of the gel.

13. The apparatus of claim 12 further comprising at least one fourth vertical wall light source attached to the inner surface of a fourth vertical wall wherein the fourth vertical wall light source is positioned to provide orthogonal illumination to a fourth vertical edge of the gel.

14. The apparatus of claim 2 further comprising at least one light guide positioned in proximity to said at least one first vertical wall light source and suitably disposed to diffuse light emitted by the first vertical wall light source evenly across an edge of the gel being viewed.

15. An apparatus useful for the visualization of fluorophores during a gel electrophoresis procedure comprising:

a vessel to perform electrophoreses procedures, the vessel including a rectangular tray or tank comprising a bottom and four walls having an inner and an outer surface and wherein the tray is dimensioned to contain an electrophoresis gel, at least first and second light sources positioned to illuminate respective first and second edges of an electrophoresis gel disposed within the vessel allowing the fluorescence emitted by the gel to be visualized by a viewer or an optical instrument suitable for such visualization from a surface of the gel that is orthogonal to the first and second edges of the gel, the first and second edges of the gel being contiguous and orthogonal to each other.

16. The apparatus of claim 15 further comprising one or more optical filters disposed between the gel and a viewer or an optical instrument wherein that the optical filter functions to aid in detection of fluorescence emitted from a gel therein disposed.

17. An apparatus useful for the visualization of fluorophores comprising:

a vessel comprising a rectangular base having a flat inner surface and one to four vertical walls each disposed along an edge of the rectangular base, wherein each vertical wall has an inner surface and wherein the vessel is dimensioned to contain an electrophoresis gel, said gel comprising parallel planer top and bottom gel surfaces and gel edges disposed orthogonally to the plane of said top and bottom gel surfaces;

wherein

at least one vertical wall is provided with one or more light sources positioned to illuminate at least one gel edge of an electrophoresis gel disposed therein; and

wherein said one or more light sources provide one or more of the excitation wavelengths required to effect the emission of fluorescence by fluorophores contained within a gel disposed within the vessel such that the fluorophores can be visualized by a viewer or an optical instrument suitable for such visualization by directed to the top or bottom surface of the gel; and

wherein at least one vertical wall is movable with respect to an edge of a gel disposed therein such that the distance between said one or more light sources and the gel edge can be varied to provide a desired illumination intensity.

18. The apparatus of claim 17 further comprising one or more light guides positioned to direct light emitted by said one or more light sources towards one or more edges of a gel disposed therein.

19. The apparatus of claim 17, wherein the gel is rectangular and has first, second, third, and fourth edges, and the apparatus includes at least third and fourth light sources respectively configured to provide light into the third and fourth edges such that all four edges of the gel can be illuminated simultaneously.