Dual inlet microchannel device and method for using same

• US 8,974,748 B2
• Filed: 11/08/2007
• Issued: 03/10/2015
• Est. Priority Date: 04/05/2007
• Status: Active Grant

• Current Assignee: Corning Incorporated
• Original Assignee: Corning Incorporated
• Inventors: Bergman, Richard, Lewis, Mark A., Li, Cheng-Chung, Miller, William J.
• Primary Examiner(s): Hyun, Paul
• Assistant Examiner(s): EOM, ROBERT J
• Application Number:
  US11/983,327
• Publication Number:
  US 20080247907A1
• Time in Patent Office: 2,679 Days
• Field of Search: 422/102, 422/500, 422/501, 422/502, 422/503, 422/504, 422/507
• US Class Current: 422/503
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/565 Seals in general F16L
• CPC Class Codes: G01N 33/557 using kinetic measurement, i.e. time rate of progress of an antigen-antibody interaction

Abstract

A dual inlet microchannel device and a method for using the device to perform a flow-through kinetic assay are described. A microplate having an array of the dual inlet microchannel devices and in particular their specially configured flow chambers is also described. Several embodiments of the dual inlet microchannel devices and specially configured flow chambers are also described.

Claims

1. A dual inlet microchannel device comprising:

a body having formed therein;

a first fluid inlet;

a second fluid inlet;

a fluid outlet; and

a flow chamber comprising;

a first flow restrictive neck associated with the first fluid inlet;

a second flow restrictive neck associated with the second fluid inlet;

a central portion having one corner of one end associated with the first flow restrictive neck and another corner of the one end associated with the second flow restrictive neck;

an outlet portion associated with the fluid outlet and an opposite end of the central portion, wherein the opposite end is directly opposite from both an opening of the one corner and an opening of the another corner associated with the one end of the central portion;

wherein the first fluid inlet is a buffer solution inlet and the second fluid inlet is an analyte solution inlet, and wherein the first flow restrictive neck has an output opening directly opposite an output opening of the second flow restrictive neck;

wherein the flow chamber has a height of about 10 μ

m-500 μ

m, a length of about 2 mm-8 mm, and a width of about 1 mm-5 mm; and

wherein the first flow restrictive neck and the second flow restrictive neck fluid inlet each has a width of about 100 μ

m-1000 μ

m, and a length of about 200 μ

m-3000 μ

m.

2. The device of claim 1, further comprising a sensor that has a sensing surface located within the central portion of the flow chamber.

3. The device of claim 1, wherein the flow chamber further includes:

a first inlet portion located between the first fluid inlet and the first flow restrictive neck;

a second inlet portion located between the second fluid inlet and the second flow restrictive neck; and

wherein the first fluid inlet portion and the second fluid inlet portion each has a diameter of about 0.25 mm-1 mm.

4. The dual inlet microchannel device of claim 1, wherein the central portion further includes a third flow restrictive neck associated with the first flow restrictive neck and the second flow restrictive neck;

and wherein the third flow restrictive neck has a width of about 200 μ

m-1500 μ

m, and a length of about 200 μ

m-1500 μ

m.

5. A dual inlet microchannel device comprising:

a body having formed therein;

a first fluid inlet configured to receive a first solution;

a second fluid inlet configured to receive an second solution;

a fluid outlet; and

a flow chamber configured to have a fast switch time between having either the first solution or the second solution flowing therein, wherein the flow chamber comprises;

a first flow restrictive neck associated with the first fluid inlet;

a second flow restrictive neck associated with the second fluid inlet;

a central portion having one corner of one end associated with the first flow restrictive neck and another corner of the one end associated with the second flow restrictive neck;

an outlet portion associated with the fluid outlet and an opposite end of the central portion, wherein the opposite end is directly opposite from both an opening of the one corner and an opening of the another corner associated with the one end of the central portion;

wherein said flow chamber is configured such that when only the first solution is flowing therein there is a pressure drop in the first flow restrictive neck such that once the first solution leaves the first flow restrictive neck then a path of least resistance for a flow of the first solution is to the outlet portion and the fluid outlet rather than partly to the outlet portion and the fluid outlet and partly across the central portion to the second flow restrictive neck and the second fluid inlet;

wherein said flow chamber is configured such that when only the second solution is flowing therein there is a pressure drop in the second flow restrictive neck such that once the second solution leaves the second flow restrictive neck then a path of least resistance for a flow of the second solution is to the outlet portion and the fluid outlet rather than partly to the outlet portion and the fluid outlet and partly across the central portion to the first flow restrictive neck and the first fluid inlet;

wherein the first fluid inlet is a buffer solution inlet and the second fluid inlet is an analyte solution inlet, wherein the first solution is a buffer solution and the second solution is an analyte solution, and wherein the first flow restrictive neck has an output opening directly opposite an output opening of the second flow restrictive wherein the flow chamber has a height of about 10 μ

m-500 μ

m, a length of about 2 mm-8 mm, and a width of about 1 mm-5 mm; and

wherein the first flow restrictive neck and the second flow restrictive neck fluid inlet each has a width of about 100 μ

m-1000 μ

m, and a length of about 200 μ

m-3000 μ

m.

6. The dual inlet microchannel device of claim 5, further comprising a sensor that has a sensing surface located within the central portion of the flow chamber.

7. The dual inlet microchannel device of claim 5, wherein the flow chamber further includes:

a first inlet portion located between the first fluid inlet and the first flow restrictive neck;

a second inlet portion located between the second fluid inlet and the second flow restrictive neck; and

wherein the first fluid inlet portion and the second fluid inlet portion each has a diameter of about 0.25 mm-1 mm.

8. The dual inlet microchannel device of claim 5, wherein the central portion further includes a third flow restrictive neck associated with the first flow restrictive neck and the second flow restrictive neck;

and wherein the third flow restrictive neck has a width of about 200 μ

m-1500 μ

m, and a length of about 200 μ

m-1500 μ

m.

9. A dual inlet microchannel device comprising:

a body having formed therein;

a first fluid inlet;

a second fluid inlet;

a fluid outlet; and

a flow chamber comprising;

a first flow restrictive neck associated with the first fluid inlet;

a second flow restrictive neck associated with the second fluid inlet;

a central portion having one corner of one end associated with the first flow restrictive neck and another corner of the one end associated with the second flow restrictive neck;

an outlet portion associated with the fluid outlet and an opposite end of the central portion, wherein the opposite end is directly opposite from both an opening of the one corner and an opening of the another corner associated with the one end of the central portion;

the central portion further comprises a third flow restrictive neck associated with the first flow restrictive neck and the second flow restrictive neck;

the flow chamber has a height of about 10 μ

m-500 μ

m, a length of about 2 mm-8 mm, and a width of about 1 mm-5 mm;

the first flow restrictive neck and the second flow restrictive neck fluid inlet each has a width of about 100 μ

m-1000 μ

m, and a length of about 200 μ

m-3000 μ

m; and

the third flow restrictive neck has a width of about 200 μ

m-1500 μ

m, and a length of about 200 μ

m-1500 μ

m.

10. The dual inlet microchannel device of claim 9, further comprising a sensor that has a sensing surface located within the central portion of the flow chamber.

11. The dual inlet microchannel device of claim 9, wherein the flow chamber further includes:

a first inlet portion located between the first fluid inlet and the first flow restrictive neck;

a second inlet portion located between the second fluid inlet and the second flow restrictive neck; and

wherein the first fluid inlet portion and the second fluid inlet portion each has a diameter of about 0.25 mm-1 mm.