DNA analysis apparatus
First Claim
1. A DNA analysis apparatus, comprising:
- a movable stage supporting a DNA holding carrier which holds a DNA sample;
a substrate supplying part which individually supplies plural types of substrates for complementary strand synthesis to the DNA sample held by the DNA holding carrier;
a reaction vessel which accommodates a reaction solution that produces luminescence through reaction with a DNA complementary strand synthesis reaction product, wherein the reaction solution contains Apyrase that degrades the substrates;
a photodetector disposed proximal to the reaction vessel to detect luminescence produced in the reaction solution; and
a computer to control positioning of the stage and control dispensing of the substrates for complementary strand synthesis onto the DNA holding carrier from the substrate supplying part, whereinthe computer positions the stage so the DNA holding carrier is spatially separated from the reaction solution in the reaction vessel and the DNA holding carrier receives the substrates from the substrate supplying part for performing complementary strand synthesis, and then moves the stage so that the holding carrier is dipped into the reaction solution in the reaction vessel for performing a luminescence reaction and degrading the substrates by Apyrase.
1 Assignment
0 Petitions
Accused Products
Abstract
Accurate and sensitive sequencing in pyrosequencing is achieved by allowing complementary strand synthesis reaction to proceed homogeneously and completely in a short time while performing luminescence reaction for a sufficiently long time. DNA as a sequencing target is immobilized on the surface of a solid support. Nucleic acid substrates are injected from a dispenser to the support site where complementary strand synthesis is in turn performed rapidly and completely in a short time under a small reaction volume. Next, the support together with the product thereon is moved into a luminescence reaction solution where luminescence reaction is in turn performed. Thus, a DNA complementary strand synthesis reaction site and a luminescence reaction site are completely separated. The support surface is also washed by dipping the support in the luminescence reaction solution that contains a luminescence reagent and an enzyme that degrades redundant nucleic acid substrates.
12 Citations
11 Claims
-
1. A DNA analysis apparatus, comprising:
-
a movable stage supporting a DNA holding carrier which holds a DNA sample; a substrate supplying part which individually supplies plural types of substrates for complementary strand synthesis to the DNA sample held by the DNA holding carrier; a reaction vessel which accommodates a reaction solution that produces luminescence through reaction with a DNA complementary strand synthesis reaction product, wherein the reaction solution contains Apyrase that degrades the substrates; a photodetector disposed proximal to the reaction vessel to detect luminescence produced in the reaction solution; and a computer to control positioning of the stage and control dispensing of the substrates for complementary strand synthesis onto the DNA holding carrier from the substrate supplying part, wherein the computer positions the stage so the DNA holding carrier is spatially separated from the reaction solution in the reaction vessel and the DNA holding carrier receives the substrates from the substrate supplying part for performing complementary strand synthesis, and then moves the stage so that the holding carrier is dipped into the reaction solution in the reaction vessel for performing a luminescence reaction and degrading the substrates by Apyrase. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
Specification
-
- Resources
-
Current AssigneeHitachi, Ltd.
-
Original AssigneeHitachi, Ltd.
-
InventorsKambara, Hideki, Kishimoto, Akihiko
-
Primary Examiner(s)HOBBS, MICHAEL L
-
Application NumberUS12/419,399Publication NumberTime in Patent Office2,163 DaysField of Search435/6, 435/287.2, 422/82.08US Class Current435/287.2CPC Class CodesB01J 2219/00659 Two-dimensional arraysB01J 2219/00722 NucleotidesB01L 7/52 with provision for submitti...C12Q 1/6869 Methods for sequencingC12Q 2535/101 Sanger sequencing method, i...C12Q 2563/103 luminescenceC12Q 2565/301 Pyrophosphate (PPi)
