Isolated stem cell comprising a Xic flanking region transgene
First Claim
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1. An isolated stem cell comprising an Xic flanking region transgene having a polynucleotide sequence at least 95% identical to the polynucleotide sequence of SEQ ID NO:
- 47, or a fragment thereof encoding a protein that prevents the stem cells from undergoing X chromosome inactivation and from differentiating in culture, wherein expression of the flanking region transgene inhibits the stem cell from undergoing X chromosome inactivation and differentiation in culture.
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Abstract
Disclosed herein are methods for controlling stem cell differentiation through the introduction of transgenes having Xic, Tsix, Xite, or Xic flanking region sequences to block differentiation and the removal of the transgenes to allow differentiation. Also disclosed are small RNA molecules and methods for using the small RNA molecules to control stem cell differentiation. Also disclosed are stem cells genetically modified by the introduction of Xic, Tsix, XUe, or Xic flanking region sequences.
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12 Claims
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1. An isolated stem cell comprising an Xic flanking region transgene having a polynucleotide sequence at least 95% identical to the polynucleotide sequence of SEQ ID NO:
- 47, or a fragment thereof encoding a protein that prevents the stem cells from undergoing X chromosome inactivation and from differentiating in culture, wherein expression of the flanking region transgene inhibits the stem cell from undergoing X chromosome inactivation and differentiation in culture.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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