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Method and device for identifying multipotent stem cell colony, and method and device for automatically culturing multipotent stem cells

  • US 8,977,031 B2
  • Filed: 06/22/2011
  • Issued: 03/10/2015
  • Est. Priority Date: 06/25/2010
  • Status: Expired due to Fees
First Claim
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1. A method for identifying a colony comprising identifying an undifferentiated colony and an undefined colony based on an image taken inside of an incubator of multipotent stem cells, wherein, when a predetermined first threshold of a circularity of the colony is C1 and a second threshold of the circularity of the colony is C2 (C1<

  • C2), a circularity C of the colony obtained from the image is obtained by calculation, anda colony satisfyingC2

    Cis determined as the undifferentiated colony containing only undifferentiated multipotent stem cells, anda colony satisfyingC1

    C<

    C2is determined as an undefined colony that is potentially the undifferentiated colony.

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