Method and device for identifying multipotent stem cell colony, and method and device for automatically culturing multipotent stem cells
First Claim
1. A method for identifying a colony comprising identifying an undifferentiated colony and an undefined colony based on an image taken inside of an incubator of multipotent stem cells, wherein, when a predetermined first threshold of a circularity of the colony is C1 and a second threshold of the circularity of the colony is C2 (C1<
- C2), a circularity C of the colony obtained from the image is obtained by calculation, anda colony satisfyingC2≦
Cis determined as the undifferentiated colony containing only undifferentiated multipotent stem cells, anda colony satisfyingC1≦
C<
C2is determined as an undefined colony that is potentially the undifferentiated colony.
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Abstract
A method and device achieves identification on whether a colony on a cultivation container is differentiated or undifferentiated on an occasion of cultivation of multipotent stem cell, and a method and device achieves a rapid culture of undifferentiated multipotent stem cells. Undifferentiated colony containing only undifferentiated multipotent stem cells is identified from other types of colonies by means of the circularity C of the colony in an image-processed taken image of the inside of cultivation container of a multipotent stem cell. More specifically, when a first threshold of the circularity of the colony is C1, and a second predetermined threshold is C2 (C1<C2), a colony satisfying C2≦C is determined as the undifferentiated colony, a colony satisfying C1≦C<C2 is determined as an undefined colony, and a colony satisfying C<C1 is determined as the differentiated colony.
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Citations
19 Claims
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1. A method for identifying a colony comprising identifying an undifferentiated colony and an undefined colony based on an image taken inside of an incubator of multipotent stem cells, wherein, when a predetermined first threshold of a circularity of the colony is C1 and a second threshold of the circularity of the colony is C2 (C1<
- C2), a circularity C of the colony obtained from the image is obtained by calculation, and
a colony satisfying C2≦
Cis determined as the undifferentiated colony containing only undifferentiated multipotent stem cells, and a colony satisfying C1≦
C<
C2is determined as an undefined colony that is potentially the undifferentiated colony. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- C2), a circularity C of the colony obtained from the image is obtained by calculation, and
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11. A device for identifying colonies, comprising:
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an image acquisition means for acquiring an image taken inside of an incubator of multipotent stem cells; and an identification means for identifying an differentiated colony and an undifferentiated colony based on a circularity C of the colony obtained from the image, wherein, when a predetermined first threshold of the circularity of the colony is C1, and a second predetermined threshold of the circularity of the colony is C2 (C1<
C2), the identification means determines that;a colony satisfying C2≦
Cis the undifferentiated colony containing only undifferentiated multipotent stem cells, a colony satisfying C1≦
C<
C2is an undefined colony that is potentially an undifferentiated colony, and a colony satisfying C<
C1is the differentiated colony containing multipotent stem cells. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
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Specification