Multiple wavelength LED array illuminator for fluorescence microscopy

• US 8,979,302 B2
• Filed: 11/04/2013
• Issued: 03/17/2015
• Est. Priority Date: 04/15/2011
• Status: Active Grant

• Current Assignee: DiCon Fiber Optics Incorporated
• Original Assignee: DiCon Fiber Optics Incorporated
• Inventors: Lee, Jeffrey B., Lu, Junying Jonathan, Schleicher, Robert E.
• Primary Examiner(s): Ton, Anabel
• Application Number:
  US14/071,334
• Publication Number:
  US 20140063797A1
• Time in Patent Office: 498 Days
• Field of Search: 362/231, 362/268, 362/249.02, 359/385
• US Class Current: 362/231
• CPC Class Codes: F21K 9/60 Optical arrangements integrated in the light source, e.g. for improving the colour rendering index or the light extraction
• CPC Class Codes: F21V 9/00 Elements for modifying spectral properties, polarisation or intensity of the light emitted, e.g. filters coloured shades F21V1/00; elements characterised by cooling arrangements F21V29/502
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 26/008 in the form of devices for effecting sequential colour changes, e.g. colour wheels

Abstract

One embodiment provides light along an optical axis. It comprises a substrate and at least one array of multiple LED chips without individual packaging supported by the substrate. The LED chips emit light within different wavelength ranges and are distributed laterally with respect to the axis over an area, the LED chips having light emitting surfaces for emitting light in directions transverse to the area. An optical element adjacent to the light emitting surfaces of the LED chips in the at least one array collects and directs light emitted by the LED chips of the at least one array along the axis towards a target. Another embodiment is directed to a method for providing multiple wavelength light for fluorescent microscopy using the above system. Electric current is supplied to the multiple LED chips, causing them to emit light of multiple wavelengths. The currents supplied to the multiple LED chips are controlled so as to control the exposure of fluorescent dyes with different excitation wavelengths wherein the light emitted by the multiple LED chips include wavelength components at such different excitation wavelengths without having to move the multiple LED chips.

Claims

1. A LED array spot illuminator for providing light along an optical axis, comprising:

a substrate;

at least one array of multiple LED chips without individual packaging supported by said substrate, wherein the LED chips emit light within different wavelength ranges and are distributed laterally with respect to said axis over a light-emitting area, said LED chips having light emitting surfaces for emitting light in directions transverse to said area,

an optical element adjacent to the light emitting surfaces of the LED chips in said at least one array and in the optical axis collecting light emitted by the LED chips;

an optical device that collects and directs light emitted by the LED chips of the at least one array and collected by said optical element along the optical axis;

an aperture located in the optical axis, wherein light collected by said optical element and said optical device and passed by the aperture forms a beam of light illuminating a spot; and

a diffusing/scattering/homogenizing element in the optical axis to homogenize light within different wavelength ranges so that the beam illuminating the spot is substantially spectrally uniform across the surface of the illuminated spot.

2. The illuminator of claim 1, wherein the diffusing/scattering/homogenizing element is located between the at least one array of multiple LED chips and the aperture.

3. The illuminator of claim 1, wherein the dimensions of the light-emitting area do not exceed 25 mm.

4. The illuminator of claim 1, wherein the optical element and optical device are in the optical axis, said illuminator further comprising a device for adjusting positions along an optical axis of the optical element and optical device.

5. The illuminator of claim 1, further comprising a device for adjusting positions along an optical axis of the aperture and diffusing/scattering/homogenizing element.

6. The illuminator of claim 5, wherein the device adjusts positions along an optical axis of the optical device, the aperture and diffusing/scattering/homogenizing element simultaneously.

7. The illuminator of claim 1, wherein the optical element comprises a half-ball lens.

8. The illuminator of claim 1, further comprising a mirror or a light guide for directing the light from the optical element to the target.

9. The illuminator of claim 8, the light guide comprising embedded diffusing elements.

10. The illuminator of claim 1, further comprising an electronic control circuit for supplying electric current to the at least one array of multiple LED chips, and at least one interface for receiving computer or user commands for controlling the electric current supplied by the circuit to control light emission by the multiple LED chips.