Biosensor, thin film electrode forming method, quantification apparatus, and quantification method
First Claim
1. A biosensor for quantifying a substrate in a sample liquid, the biosensor comprising:
- an insulating support;
an electrode pattern and an identification pattern formed of an electrically conductive material on a surface of the insulating support and having a thickness from 3 nm to 100 nm;
a reagent layer covering a portion of the electrode pattern;
a spacer layer covering a portion of the reagent layer; and
a cover on the spacer layer;
wherein;
the spacer layer defines a supply path for bringing the sample liquid into contact with the reagent layer;
a portion of the reagent layer is in the supply path, and the supply path is narrower than the reagent layer;
the electrically conductive material comprises a noble metal;
the electrode pattern defines at least a working electrode and a counter electrode;
a portion of the working electrode and a portion of the counter electrode are in the supply path; and
no portion of the identification pattern is in the supply path.
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Accused Products
Abstract
A biosensor is disclosed comprising a support; a conductive layer composed of an electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.
113 Citations
23 Claims
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1. A biosensor for quantifying a substrate in a sample liquid, the biosensor comprising:
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an insulating support; an electrode pattern and an identification pattern formed of an electrically conductive material on a surface of the insulating support and having a thickness from 3 nm to 100 nm; a reagent layer covering a portion of the electrode pattern; a spacer layer covering a portion of the reagent layer; and a cover on the spacer layer; wherein; the spacer layer defines a supply path for bringing the sample liquid into contact with the reagent layer; a portion of the reagent layer is in the supply path, and the supply path is narrower than the reagent layer; the electrically conductive material comprises a noble metal; the electrode pattern defines at least a working electrode and a counter electrode; a portion of the working electrode and a portion of the counter electrode are in the supply path; and no portion of the identification pattern is in the supply path. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification