Oncolytic vaccinia virus cancer therapy
First Claim
1. A method of inducing oncolysis in a human subject having a tumor comprising administering to said subject one or more single doses of a phannaceutical composition comprising a pharmaceutically acceptable carrier and a purified TK-deficient, human GM-CSF-expressing, replication-competent Wyeth strain vaccinia virus vector wherein said single dose comprises between 1×
- 109 pfu and 1×
1010 pfu, and wherein said administering comprises injection into the tumor mass or tumor vasculature, whereby oncolysis is induced in said tumor and in at least one tumor that is not injected with the virus.
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Abstract
Embodiments of the invention are directed methods that include a thymidine kinase deficient vaccinia virus. The methods include administering the vaccinia virus at increased viral concentrations. Further aspects of the invention include methods for inducing oncolysis or collapse of tumor vasculature in a subject having a tumor comprising administering to a subject at least 1×108 infectious viral particles of a TK-deficient, GM-CSF-expressing, replication-competent vaccinia virus vector sufficient to induce oncolysis of cells in the tumor.
157 Citations
20 Claims
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1. A method of inducing oncolysis in a human subject having a tumor comprising administering to said subject one or more single doses of a phannaceutical composition comprising a pharmaceutically acceptable carrier and a purified TK-deficient, human GM-CSF-expressing, replication-competent Wyeth strain vaccinia virus vector wherein said single dose comprises between 1×
- 109 pfu and 1×
1010 pfu, and wherein said administering comprises injection into the tumor mass or tumor vasculature, whereby oncolysis is induced in said tumor and in at least one tumor that is not injected with the virus. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
- 109 pfu and 1×
Specification