Optical determination of pH and glucose
First Claim
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1. A method for simultaneously determining pH and glucose concentration using a single fluorescent dye, comprising:
- providing a system comprising;
an optical sensor, comprising an indicator system, comprising;
a fluorescent dye that exists in first and second different forms depending on pH, wherein the different forms can be distinguished based on their respective first and second emissions; and
a binding moiety that binds glucose, wherein the binding moiety is operably coupled to the fluorescent dye, and wherein binding of glucose by the binding moiety causes an optical change in the apparent concentration of the fluorescent dye related to the concentration of glucose;
wherein a ratio of the first and second emissions is independent of the concentration of glucose;
at least one light source;
at least one detector configured to detect both the first and second emissions; and
a controller configured to monitor signals from the at least one detector and determine the ratio;
inserting the optical sensor into a tissue comprising interstitial fluid;
irradiating the sensor using the at least one light source;
detecting the first emission corresponding to the first form of the fluorescent dye;
detecting the second emission corresponding to the second form of the fluorescent dye;
ratiometrically determining the pH of the interstitial fluid using the controller; and
determining the glucose concentration in the interstitial fluid corrected for pH using the controller.
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Abstract
Embodiments of the present invention are directed to an optical sensor capable of measuring two analytes simultaneously with a single indicator system. In preferred embodiments, the sensor comprises a fluorescent dye having acid and base forms that facilitate ratiometric pH sensing, wherein the dye is further associated with a glucose binding moiety and configured to generate a signal that varies in intensity with the concentration of glucose.
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18 Claims
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1. A method for simultaneously determining pH and glucose concentration using a single fluorescent dye, comprising:
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providing a system comprising; an optical sensor, comprising an indicator system, comprising; a fluorescent dye that exists in first and second different forms depending on pH, wherein the different forms can be distinguished based on their respective first and second emissions; and a binding moiety that binds glucose, wherein the binding moiety is operably coupled to the fluorescent dye, and wherein binding of glucose by the binding moiety causes an optical change in the apparent concentration of the fluorescent dye related to the concentration of glucose; wherein a ratio of the first and second emissions is independent of the concentration of glucose; at least one light source; at least one detector configured to detect both the first and second emissions; and a controller configured to monitor signals from the at least one detector and determine the ratio; inserting the optical sensor into a tissue comprising interstitial fluid; irradiating the sensor using the at least one light source; detecting the first emission corresponding to the first form of the fluorescent dye; detecting the second emission corresponding to the second form of the fluorescent dye; ratiometrically determining the pH of the interstitial fluid using the controller; and determining the glucose concentration in the interstitial fluid corrected for pH using the controller. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method for simultaneously determining the concentration of two or more analytes using a single fluorescent dye, comprising:
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providing a system comprising; an optical sensor, comprising an indicator system, comprising; a fluorescent dye that exists in first and second different forms depending on the concentration of a first analyte, wherein the different forms can be distinguished based on their respective first and second emissions; and a binding moiety that binds a second analyte, wherein the binding moiety is operably coupled to the fluorescent dye, and wherein binding of the second analyte by the binding moiety causes an optical change in the apparent concentration of the fluorescent dye related to a concentration of the second analyte; wherein a ratio of the first and second emissions is independent of the concentration of the second analyte; at least one light source; at least one detector configured to detect both the first and second emissions; and a controller configured to monitor signals from the at least one detector and determine the ratio; inserting the optical sensor into a tissue comprising interstitial fluid; irradiating the sensor using the at least one light source; detecting the first emission corresponding to the first form of the fluorescent dye; detecting the second emission corresponding to the second form of the fluorescent dye; ratiometrically determining the concentration of the first analyte of the interstitial fluid using the controller; and determining the concentration of the second analyte in the interstitial fluid corrected for concentration of the first analyte using the controller.
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Specification