Formation of immobilized biological layers for sensing

• US 8,986,526 B2
• Filed: 12/12/2012
• Issued: 03/24/2015
• Est. Priority Date: 12/20/2007
• Status: Active Grant

• Current Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Collier, Gordon Bruce, Macleod, Jason Andrew, Wong, Anjulia, Nemeth, Attila Csaba
• Primary Examiner(s): Van, Luan
• Assistant Examiner(s): Kessel, Maris R
• Application Number:
  US13/711,886
• Publication Number:
  US 20130112558A1
• Time in Patent Office: 832 Days
• Field of Search: 20440301-40315, 205/775, 205/777.5, 205/789, 205792-793, 205/778
• US Class Current: 204/403.1
• CPC Class Codes: B05C 11/1034 specially designed for conducting intermittent application of small quantities, e.g. drops, of coating material B05C5/0225, B05C11/1026 take precedence
• CPC Class Codes: B05C 5/0291 the material being discharged on the work through discrete orifices as discrete droplets, beads or strips that coalesce on the work or are spread on the work so as to form a continuous coating
• CPC Class Codes: B05C 9/12 the auxiliary operation being performed after the application B05C9/14 takes precedence
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: C12Q 1/003 Functionalisation
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/42 involving phosphatase
• CPC Class Codes: C12Q 1/58 involving urea or urease

Abstract

The invention is directed to enzyme immobilization compositions comprising: one or more enzymes, a humectant, an acrylic-based monomer, a water-soluble organic photo-initiator and a water-soluble acrylic-based cross-linker in a substantially homogeneous aqueous mixture. The invention is also directed to methods for forming sensors comprising such compositions and to apparati for forming arrays of immobilized layers on an array of sensors by dispensing such compositions onto a substrate.

Claims

1. A sensor, comprising an electrode with a first layer covering said electrode comprising an ion-selective membrane, and a second layer covering said ion-selective membrane comprising a UV cured matrix formed from a homogeneous aqueous mixture of one or more enzymes, a humectant, an acrylic-based monomer, a water-soluble organic photo-initiator and a water-soluble acrylic-based cross-linker.

2. The sensor of claim 1, wherein said enzyme is selected from urease, glucose oxidase, lactate oxidase, creatinase, creatininase, sarcosine oxidase, catalase, carbonic anhydrase, NAD(P)H oxidase, cholesterol oxidase, alcohol oxidase, choline oxidase, glycerol-3-phosphate oxidase, thiamine oxidase, pyruvate oxidase, pyridoxal oxidase, D-amino acid oxidase, L-amino acid oxidase, alkaline phosphatase, horseradish peroxidase and combinations thereof.

3. The sensor of claim 1, wherein the monomer is selected from acrylamide, methacrylamide, N-[3-(dimethylamino)propyl]methacrylamide, hydroxyethylmethacrylate and combinations thereof.

4. The sensor of claim 1, wherein the cross-linker is selected from 1,4-bisacryloyl piperazine, N,N′

(1,2-dihydroxyethylene)bis-acrylamide,N,N′

-bis(acryloyl)cystamine, N,N′

-methylenebisacrylamide, ethylene glycol diacrylate, (+)—

N,N′

-diallyltartramide and combinations thereof.

5. The sensor of claim 1, wherein the enzyme is urease and the ion-selective membrane is an ammonium ion-selective membrane.