Thermostable DNA polymerase

• US 8,986,968 B2
• Filed: 02/13/2009
• Issued: 03/24/2015
• Est. Priority Date: 02/28/2008
• Status: Active Grant

• Current Assignee: Genesys Biotech Ltd.
• Original Assignee: Genesys Biotech Ltd.
• Inventors: Clark, Duncan, Morant, Nicholas
• Primary Examiner(s): HUTSON, RICHARD G
• Application Number:
  US12/919,622
• Publication Number:
  US 20110008848A1
• Time in Patent Office: 2,230 Days
• Field of Search: None
• US Class Current: 435/194
• CPC Class Codes: C12N 9/1252 DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Abstract

There is provided a polypeptide having thermostable DNA polymerase activity and comprising or consisting of an amino acid sequence with at least 55% identity to Thermodesulfatator indicus DNA polymerase I Large fragment shown in SEQ ID NO: 1 or in SEQ ID NO:32.

Claims

1. An isolated polypeptide comprising artificially introduced amino acids at the N terminus and having at least 80% sequence identity to the amino acid sequence of SEQ ID NO:

1, wherein said polypeptide has thermostable DNA polymerase activity and exhibits strand displacement activity.

2. The isolated polypeptide of claim 1, wherein said polypeptide catalyses an isothermal amplification reaction and/or catalyses a thermocycling amplification reaction.

3. The isolated polypeptide of claim 1, wherein said polypeptide has at least 80% sequence identity to SEQ ID NO:

32.

5. The isolated polypeptide of claim 1, wherein said polypeptide further comprises a Cren7 enhancer domain.

6. A composition comprising the isolated polypeptide of claim 1.

7. A kit comprising the isolated polypeptide of claim 1, and packaging materials therefor.

8. A kit comprising the composition of claim 6 and packaging materials therefor.

9. The isolated polypeptide of claim 1, wherein said polypeptide has at least 85% sequence identity to the amino acid sequence of SEQ ID NO:

1.

10. The isolated polypeptide of claim 9, wherein said polypeptide has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:

1.

11. The isolated polypeptide of claim 10, wherein said polypeptide has at least 95% sequence identity to the amino acid sequence of SEQ ID NO:

1.

12. The isolated polypeptide of claim 1, wherein said polypeptide is obtained from a nucleic acid modified for expression in a host cell.

13. The isolated polypeptide of claim 12, wherein the nucleic acid is SEQ ID NO:

3 or SEQ ID NO;

33.

14. The isolated polypeptide of claim 1, wherein the artificially introduced amino acids at the N terminus comprise a dipeptide.

15. The isolated polypeptide of claim 14, wherein the dipeptide is MG.

4. The isolated polypeptide of 1, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:

32.