Dual polarization with liquid crystal tunable filters

• US 8,988,680 B2
• Filed: 10/15/2012
• Issued: 03/24/2015
• Est. Priority Date: 04/30/2010
• Status: Active Grant

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Nelson, Matthew
• Primary Examiner(s): Nur, Abdullahi
• Application Number:
  US13/651,600
• Publication Number:
  US 20130038877A1
• Time in Patent Office: 890 Days
• Field of Search: 356/300, 356/326, 356/328, 356/317, 356/417
• US Class Current: 356/326
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0224 using polarising or depolarising elements
• CPC Class Codes: G01J 3/32 Investigating bands of a spectrum in sequence by a single detector
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01N 21/21 Polarisation-affecting properties G01N21/19 takes precedence
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems
• CPC Class Codes: G01N 21/314 with comparison of measurements at specific and non-specific wavelengths dual wavelength spectrometry G01J3/427
• CPC Class Codes: G01N 21/33 using ultraviolet light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/3581 using far infrared light; using Terahertz radiation
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G02B 21/0092 Polarisation microscopes
• CPC Class Codes: G02B 21/16 adapted for ultraviolet illumination ; Fluorescence microscopes G02B21/0076 takes precedence
• CPC Class Codes: G02B 21/361 Optical details, e.g. image relay to the camera or image sensor G02B21/364 takes precedence; illumination details G02B21/06 and subgroups

Abstract

A high passband transmission ratio is obtained by splitting a light beam from an objective lens into two orthogonally-polarized components processed along distinct paths through two independently controllable liquid crystal tunable filters (LCTFs). The filtered portions may be combined at an imaging plane or may be separately processed without recombining. Using two LCTFs, the arrangements discussed herein may ideally achieve 100% transmission in a single passband when two orthogonal components of a single wavelength are tuned, or 50% transmission at two distinct passbands when two orthogonal components from two different wavelengths (one component from each wavelength) are tuned. The dual polarization configuration described herein may be used to improve contrast or detected signal intensity in various microscopy and spectroscopic/chemical imaging applications and to increase the speed of detection.

Claims

1. An optical filter unit comprising:

a first polarization assembly configured to;

(i) receive a first optical signal comprising optical components in a plurality of polarization alignments,

(ii) separate the first optical signal into a first optical component and a second optical component, and

(iii) transmit the first optical component with a first polarization alignment and the second optical component with a second polarization alignment;

a first optical filter configured to receive the first optical component and transmit at least a portion of the first optical component having a first wavelength;

a second optical filter configured to receive the second optical component and transmit at least a portion of the second optical component having a second wavelength; and

at least one detector configured to detect the first optical component and the second optical component.

2. The optical filter unit of claim 1, further comprising a first detector configured to detect the first optical component and a second detector configured to detect a second optical component.

3. The optical filter unit of claim 1, wherein the detector is configured to detect both the first optical component and the second optical component.

4. The optical filter unit of claim 1, further comprising a display configured to display the first optical component and the second optical component in an overlaid configuration.

5. The optical filter unit of claim 1, further comprising a display configured to display the first optical component and the second optical component in a non-overlaid configuration.

6. The optical filter unit of claim 1, wherein the first wavelength and the second wavelength are the same.

7. The optical filter unit of claim 1, wherein the first wavelength and the second wavelength are different.

8. The optical filter unit of claim 1, further comprising a second polarization assembly configured to combine the first optical component and the second optical component.

9. The optical filter unit of claim 1, wherein at least one of the first optical filter and the second optical filter is rotated to match orthogonally polarized light transmitted from the first polarization assembly.

10. The optical filter unit of claim 1, wherein at least one of the first optical filter and the second optical filter further comprise a tunable filter.

11. The optical filter unit of claim 10, wherein the tunable filter further comprises one or more of a liquid crystal tunable filter, a multi-conjugate liquid crystal tunable filter, an acousto-optical tunable filter, a Lyot liquid crystal tunable filter, an Evans split-element liquid crystal tunable filter, a Solc liquid crystal tunable filter, a ferroelectric liquid crystal tunable filter, a Fabry Perot liquid crystal tunable filter, and combinations thereof.

12. The optical filter unit of claim 1, wherein at least one of the first optical filter and the second optical filter further comprise a fixed filter.

13. The optical filter unit of claim 1, wherein the at least one detector comprises one or more of a CCD, a CMOS detector, an InGaAs detector, a PtSi detector, an InSb detector, a HgCdTe detector, and combinations thereof.

14. The optical filter unit of claim 1, wherein the first optical component and the second optical component are detected simultaneously.

15. The optical filter unit of claim 1, wherein the first optical component and the second optical component are detected sequentially.

16. The optical filter unit of claim 1, further comprising at least one additional optical filter and at least one additional detector configured to detect additional optical components from the optical signal.

17. An optical filter unit comprising:

a first polarization assembly configured to;

(i) receive a first optical signal comprising optical components in a plurality of polarization alignments,

(ii) separate the first optical signal into a first optical component and a second optical component, and

(iii) transmit the first optical component with a first polarization alignment and the second optical component with a second polarization alignment;

a first optical filter configured to receive the first optical component and transmit at least a portion of the first optical component having a first wavelength;

a second optical filter configured to receive the second optical component and transmit at least a portion of the second optical component having a second wavelength, wherein the second wavelength is different from the first wavelength;

at least one detector configured to detect the first optical component and the second optical component, wherein the first optical component and the second optical component are detected simultaneously; and

a display configured to display the first optical component and the second optical component in a non-overlaid configuration.

18. A method comprising:

illuminating a sample to generate interacted photons;

collecting the interacted photons to generate an optical signal;

separating the optical signal into a first optical component and a second optical component;

transmitting the first optical component to a first optical filter and the second optical component to a second optical filter;

spectrally filtering the first optical component and the second optical component in at least one passband as a function of a polarization alignment to generate a corresponding filtered component; and

detecting the filtered components to generate at least one wavelength-specific spectral image of the sample.

19. The method of claim 18, wherein the first optical filter and the second optical filter each comprise a tunable filter where each filter is independently controllable, and wherein the method further comprises controlling the first optical filter and the second optical filter to a same corresponding passband wavelength.

20. The method of claim 18, wherein the first optical filter and the second optical filter each comprise a tunable filter where each filter is independently controllable, and wherein the method further comprises controlling the first optical filter and the second optical filter to a different corresponding passband wavelength.

21. The method of claim 18, wherein at least one of the first optical filter and the second optical filter comprise a tunable filter.

22. The method of claim 21, wherein the tunable filter comprises one or more of a liquid crystal tunable filter, a multi-conjugate liquid crystal tunable filter, an acousto-optical tunable filter, a Lyot liquid crystal tunable filter, an Evans split-element liquid crystal tunable filter, a Sole liquid crystal tunable filter, a ferroelectric liquid crystal tunable filter, a Fabry Perot liquid crystal tunable filter, and combinations thereof.

23. The method of claim 21, wherein the first optical component and the second optical component are detected simultaneously.

24. The method of claim 18, wherein the first optical component and the second optical component are detected sequentially.

25. The method of claim 18, wherein the wavelength-specific spectral image comprises at least one wavelength-specific spectral image of the first optical component and at least one wavelength-specific spectral image of the second optical component.

26. The method of claim 25, further comprising displaying the at least one wavelength-specific spectral image of the first optical component and the at least one wave-length specific spectral image of the second optical component in an overlaid configuration.

27. The method of claim 25, further comprising displaying the at least one wavelength-specific spectral image of the first optical component and the at least one wave-length specific spectral image of the second optical component in non-overlaid configuration.

28. The method of claim 21, further comprising generating at least one wavelength-specific image representative of a plurality of additional optical components associated with the optical signal.