Microfluidic devices and methods of use

• US 8,992,858 B2
• Filed: 08/21/2007
• Issued: 03/31/2015
• Est. Priority Date: 10/03/2000
• Status: Expired due to Fees

• Current Assignee: California Institute of Technology
• Original Assignee: National Institutes of Health (NIH) (Government of the United States of America)
• Inventors: Chou, Hou-Pu, Fu, Anne Y., Quake, Stephen R.
• Primary Examiner(s): KWAK, DEAN P
• Application Number:
  US11/842,731
• Publication Number:
  US 20080050283A1
• Time in Patent Office: 2,779 Days
• Field of Search: 422/100, 436/180
• US Class Current: 422/503
• CPC Class Codes: A61M 2206/22 eliminating pulsatile flows, e.g. by the provision of a dampening chamber
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: F04B 11/00 Equalisation of pulses, e.g. by use of air vessels; Counteracting cavitation
• CPC Class Codes: F04B 19/006 Micropumps F04B43/043 and F04B43/095 take precedence
• CPC Class Codes: F04B 43/00 Machines, pumps, or pumping installations having flexible working members pumps or pumping installations specially adapted for elastic fluids F04B45/00
• CPC Class Codes: F04B 43/043 Micropumps
• CPC Class Codes: F04B 43/14 having plate-like flexible members
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1028 Sorting particles
• CPC Class Codes: G01N 2030/205 Diaphragm valves, e.g. deformed member closing the passage
• CPC Class Codes: G01N 2030/322 : pulse dampers
• CPC Class Codes: G01N 30/6095 : Micromachined or nanomachined, e.g. micro- or nanosize
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A microfluidic device comprises pumps, valves, and fluid oscillation dampers. In a device employed for sorting, an entity is flowed by the pump along a flow channel through a detection region to a junction. Based upon an identity of the entity determined in the detection region, a waste or collection valve located on opposite branches of the flow channel at the junction are actuated, thereby routing the entity to either a waste pool or a collection pool. A damper structure may be located between the pump and the junction. The damper reduces the amplitude of oscillation pressure in the flow channel due to operation of the pump, thereby lessening oscillation in velocity of the entity during sorting process. The microfluidic device may be formed in a block of elastomer material, with thin membranes of the elastomer material deflectable into the flow channel to provide pump or valve functionality.

Claims

1. A microfluidic device comprising:

a flow channel formed in an elastomeric material;

a pump configured to move fluid through the flow channel; and

a damper positioned along the flow channel downstream from the pump;

wherein the damper comprises one or more sealed chambers contained within the elastomeric material that each have no inlet, outlet, or valve,

wherein the sealed chamber(s) overlie and are separated from the flow channel by a membrane that is configured to deflect outwards from the flow channel into the sealed chamber(s), thereby reducing or eliminating oscillations caused by the pump in pressure of a fluid moving through the flow channel.

2. The microfluidic device of claim 1, wherein the sealed chamber(s) contain a liquid.

3. The microfluidic device of claim 1, wherein the sealed chamber(s) contain a gas.

4. The microfluidic device of claim 1, wherein the sealed chamber(s) are located above the flow channel.

5. The microfluidic device of claim 1, wherein the sealed chamber(s) are each at least about 1.5 times the width of the flow channel.

6. The microfluidic device of claim 1, comprising elastomer material on one or more side walls of the flow channel that are deflectable to further reduce or eliminate oscillations in pressure of a fluid moving through the flow channel.

7. The microfluidic device of claim 1, wherein the damper comprises at least three such sealed chambers downstream from the pump and in close proximity with each other.

8. The microfluidic device of claim 1, wherein there is a constriction in width of the flow channel downstream from the damper.

9. The microfluidic device of claim 1, wherein there is a detector configured to determine substances in the flow channel downstream from the damper.

10. The microfluidic device of claim 1, wherein there is a sorting means downstream from the damper that diverts materials passing along the flow channel in the fluid into any one of at least two branched channels.

11. The microfluidic device of claim 1, wherein the pump is a peristaltic pump.

12. The microfluidic device of claim 1, further comprising a constriction in width of the flow channel downstream from the damper.