All-in-one means of separating blood components

• US 8,992,862 B2
• Filed: 11/15/2012
• Issued: 03/31/2015
• Est. Priority Date: 04/03/2009
• Status: Active Grant

• Current Assignee: Biomet Manufacturing, LLC (Zimmer Biomet Holdings, Inc.)
• Original Assignee: Biomet Biologics LLC (Zimmer Biomet Holdings, Inc.)
• Inventors: Leach, Michael D., Chavarria, Jason
• Primary Examiner(s): Gordon, Brian R
• Application Number:
  US13/677,897
• Publication Number:
  US 20130068676A1
• Time in Patent Office: 866 Days
• Field of Search: 422/533, 422/548, 422/559, 422/918, 422/914, 422/547, 422/549, 422/527
• US Class Current: 422/533
• CPC Class Codes: A61M 1/029 Separating blood components present in distinct layers in a container, not otherwise provided for containers for storing blood or blood components A61J1/05; sampling or analysing blood by separating blood components G01N33/491
• CPC Class Codes: A61M 2202/0415 Plasma
• CPC Class Codes: A61M 2202/0427 Platelets; Thrombocytes
• CPC Class Codes: A61M 2202/0429 Red blood cells; Erythrocytes
• CPC Class Codes: A61M 2202/0464 Cerebrospinal fluid
• CPC Class Codes: A61M 2202/10 Bone-marrow
• CPC Class Codes: B01D 17/12 Auxiliary equipment particularly adapted for use with liquid-separating apparatus, e.g. control circuits
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: B01L 3/50215 using a float to separate phases
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A separation device including a first buoy, a second buoy, a first valve, and a second valve. The first buoy is mounted to a buoy guide post and slidably mounted within a separation chamber. The second buoy is slidably mounted to the guide post and movable between a first position and a second position. The second buoy closes the first valve and opens the second valve when in the first position. The second buoy opens the first valve and closes the second valve when in the second position. The second buoy has a density such that after spinning the device for a suitable period of time a first component of the composition is isolated between the first buoy and the second buoy and a second component of the composition is isolated between the second buoy and the end of the separation chamber that is opposite to a port.

Claims

1. A device having a separation chamber for separating components of a composition according to density comprising:

a port that provides fluid communication between an interior and an exterior of said separation chamber;

a buoy guide post;

a first buoy fixedly mounted to said buoy guide post and slidably mounted within said separation chamber;

a second buoy slidably mounted to said buoy guide post and movable between a first position and a second position along said buoy guide post;

a passage defined in said buoy guide post that is in fluid communication with said port;

a first valve in said buoy guide post in fluid communication with said passage and an area of said separation chamber between said first buoy and said second buoy;

a second valve in said buoy guide post in fluid communication with said passage and an area of said separation chamber between said second buoy and an end of said separation chamber that is opposite to said port;

wherein said second buoy closes said first valve and opens said second valve when in said first position; and

wherein said second buoy opens said first valve and closes said second valve when in said second position; and

wherein said second buoy has a density such that after spinning said device for a suitable period of time a first component of said composition is isolated between said first buoy and said second buoy and a second component of said composition is isolated between said second buoy and said end of said separation chamber that is opposite to said port.

2. The device of claim 1, further comprising a plunger that is slidably movable within said separation chamber, said plunger includes a plunger base that is removably attached to a plunger handle.

3. The device of claim 2, further comprising a plunger stop that restricts movement of said plunger.

4. The device of claim 1, wherein said first buoy has a density of between about 1.070 g/ml and about 1.095 g/ml.

5. The device of claim 1, wherein said first buoy has a greater density than said second buoy.

6. The device of claim 1, wherein said second buoy has a density of between about 0.93 g/ml and about 0.955 g/ml.

7. The device of claim 1, wherein said first buoy has a top surface that faces said second buoy, said top surface is concave;

and

wherein said first buoy has a bottom surface that is opposite to said top surface, said bottom surface is convex.

8. The device of claim 1, wherein said device includes a syringe.

9. The device of claim 8, wherein said syringe is connected to said port with an extension nozzle.

10. The device of claim 8, wherein said syringe is connected directly to said port.

11. The device of claim 1, wherein said device is configured to separate components of any of the following compositions:

whole blood, blood plasma, mononuclear cells, bone marrow aspirate, spinal fluid, and fat.

12. The device of claim 1, wherein said first and said second buoys are of a density configured to isolate the following:

platelet rich plasma between said first buoy and said second buoy;

red blood cells on a side of said first buoy that is opposite to said second buoy; and

platelet poor plasma on a side of said second buoy that is opposite to said first buoy.

13. The device of claim 1, wherein said first and said second buoys are of a density configured to isolate the following:

multipotent cells between the first buoy and the second buoy;

red blood cells on a side of said first buoy that is opposite to said second buoy, and bone marrow plasma on a side of said second buoy that is opposite to said first buoy.

14. The device of claim 1, further comprising a flexible conduit that connects said passage of the buoy guide post to said port.

15. The device of claim 1, further comprising a polymeric ring that extends around a cylindrical sidewall of said second buoy.

16. The device of claim 1, further comprising a first polymeric ring and a second polymeric ring that each extend around an outer diameter of said buoy guide post, said first polymeric ring and said second polymeric ring are positioned on opposite sides of said second valve.

17. A device having a separation chamber for separating components of a composition according to density comprising:

a port that provides fluid communication of the composition from an exterior of said chamber to an interior of said chamber;

a first buoy slidably mounted within said separation chamber; and

a second buoy slidably mounted within said separation chamber;

wherein said second buoy has a density such that after spinning said device for a suitable period of time a first component of said composition is isolated between said first buoy and said second buoy and a second component of said composition is isolated between said second buoy and an end of said separation chamber that is opposite to said port;

wherein said port provides fluid communication of both said first component and said second component from said interior of said chamber to said exterior of said chamber;

a buoy guide post extending along a longitudinal axis of said separation chamber in fluid communication with said port;

wherein said first buoy and said second buoy are each slidably mounted to the buoy guide post;

a first valve in the buoy guide post;

a second valve in the buoy guide post; and

wherein said second buoy closes the first valve in the buoy guide post and opens the second valve in the buoy guide post when in a first position, said second buoy opens said first valve and closes said second valve when in a second position different from the first position.

18. The device of claim 17, wherein said first buoy is connected to said second buoy.

19. The device of claim 17, wherein said first buoy is not connected to said second buoy.

20. A device for separating components of a composition according to density comprising:

a separation chamber;

a buoy guide post within said separation chamber;

a first buoy mounted to said buoy guide post;

a second buoy mounted to said buoy guide post;

a passage defined in said buoy guide post that is in fluid communication with an exterior of said separation chamber;

a first valve in said buoy guide post; and

a second valve in said buoy guide post;

wherein;

said second buoy closes said first valve and opens said second valve when in a first position;

said second buoy opens said first valve and closes said second valve when in a second position different from the first position; and

said second buoy has a density configured such that after spinning said device for a suitable period of time a first component of said composition is isolated between said first buoy and said second buoy, and a second component of said composition is isolated between said second buoy and an end of said separation chamber.