Microdissection method and information processing system

• US 8,995,733 B2
• Filed: 04/20/2010
• Issued: 03/31/2015
• Est. Priority Date: 04/28/2009
• Status: Active Grant

• Current Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Original Assignee: Koninklijke Philips N.V.
• Inventors: Stallinga, Sjoerd, Van Dijk, Erik Martinus H. P.
• Primary Examiner(s): Patel, Nirav G
• Application Number:
  US13/266,552
• Publication Number:
  US 20120045790A1
• Time in Patent Office: 1,806 Days
• Field of Search: None
• US Class Current: 382/128
• CPC Class Codes: G01N 1/04 in the solid state, e.g. by cutting
• CPC Class Codes: G01N 1/06 providing a thin slice, e.g. microtome
• CPC Class Codes: G06T 2207/10056 Microscopic image
• CPC Class Codes: G06T 2207/20104 Interactive definition of region of interest [ROI]
• CPC Class Codes: G06T 2207/30024 Cell structures in vitro; Tissue sections in vitro
• CPC Class Codes: G06T 7/0014 using an image reference approach

Abstract

A method for use in biology, histology, and pathology includes providing a digital first image of a first slice of an object having biological material; generating a digital second image of a second slice of the object; determining a region of interest in the second image based on a region of interest in the first image; determining a region of interest in the second slice based on the region of interest in the second image; and extracting material from the region of interest in the second slice.

Claims

1. A method for diagnosing biology, histology, and pathology of an object, the method comprising the acts of:

providing the object comprising biological material and including a plurality of adjacent layers and a feature of interest extending through the plurality of adjacent layers;

providing an image of a slice of a first of the plurality of the adjacent layers cut from the object;

generating an image of a slice of a second of the plurality of adjacent layers cut from the object, a delay between cutting the slices of the first and second adjacent layers of the plurality of adjacent layers is selected from any of hours, days, weeks, months, years;

determining a region of interest in the image of the slice of the second layer based on a region of interest in the image of the slice of the first layer;

determining a region of interest in the slice of the second layer based on the region of interest in the image of the slice of the second layer; and

extracting material from the region of interest in the slice of the second layer.

2. The method as set forth in claim 1, wherein the act of determining the region of interest in the image of the slice of the second layer comprises the acts of:

determining a geometrical transformation which maps positions of features in the image of the slice of the first layer into positions of similar features in the image of the slice of the second layer; and

applying the geometrical transformation to the region of interest in the image of the slice of the first layer.

3. The method as set forth in claim 1, wherein the act of determining the region of interest in the image of the slice of the second layer comprises the act of aligning the images of the slices of the first and second layers such that at least some features in the image of the slice of the first layer project onto similar features in the image of the slice of the second layer and/or such that a contrast of a superposition of the images of the slices of the first and the second layers is maximized.

4. The method as set forth in claim 1, further comprising the acts of:

determining, based on the region of interest in the second image, a new position of a cutting tool relative to the slice of the second layer; and

moving the cutting tool relative to the slice of the second layer to the new position.

5. The method as set forth in claim 4, wherein the act of determining the new position comprises the act of consulting at least one of a look-up table and evaluating a function for relating positions in the second image to one of positions of the cutting tool relative to the slice of the second layer and information equivalent to positions of the cutting tool relative to the slice of the second layer.

6. The method of claim 1, further comprising the acts of:

storing a first slice identifier of the image of the first slice on a computer readable medium having records of a plurality of slice identifiers, each record including a slice identifier and a corresponding process identifier selected from a predefined set of process identifiers indicating a process to which respective slice identifier is to be subjected; and

enabling a user, via a user interface, to select a record from the plurality of records.

7. The method of claim 6, wherein the plurality of records are identified by slice identifiers indicating an order in which the slices were arranged before being cut from the object.

8. An apparatus for use in diagnosing biology, histology, and pathology of an object comprising biological material having a plurality of adjacent layers and a feature of interest extending through the plurality of adjacent layers, comprising:

a receiver configured to provide an image of a slice of a first of the plurality of the adjacent layers cut from the object;

an imager configured to generate an image of a slice of the second layer of a second of the plurality of adjacent layers cut from the object; and

a processor configured to;

determine a region of interest in the image of the slice of the second layer based on a region of interest in the image of the slice of the first layer, and

determine a region of interest in the slice of the second layer based on the region of interest in the image of the slice of the second layer; and

an extractor configured to extract material from the region of interest in the slice of the second layer,

wherein the imager comprises a microscope objective and the extractor comprises a laser configured to generate a laser beam for transmittal to the slice of the second layer via the microscope objective,

wherein the extractor comprises a cutting tool configured to cut material out of the slice of the second layer, and the processor is further configured to;

determine, based on the region of interest in the second image, a new position of the cutting tool relative to the slice of the second layer,

move the cutting tool relative to the slice of the second layer to the new position, and

wherein the slice of the second layer is cut after a delay between cutting the slices of the first and second adjacent layers of the plurality of adjacent layers that is selected from any of hours, days, weeks, months, years.

9. A non-transitory computer readable medium comprising computer instructions which, when executed by a processor, configure the processor to perform the method for diagnosing biology, histology, and pathology of an object, the method comprising the acts of:

providing the object comprising biological material and including a plurality of adjacent layers and a feature of interest extending through the plurality of adjacent layers;

providing an image of a slice of a first of the plurality of the adjacent layers cut from the object;

generating an image of a slice of a second of the plurality of adjacent layers cut from the object, a delay between cutting the slices of the first and second adjacent layers of the plurality of adjacent layers is selected from any of hours, days, weeks, months, years;

determining a region of interest in the image of the slice of the second layer based on a region of interest in the image of the slice of the first layer;

determining a region of interest in the slice of the second layer based on the region of interest in the image of the slice of the second layer; and

extracting material from the region of interest in the slice of the second layer.

10. An information processing system, for use in biology, histology and pathology, configured for

providing a predefined set of process identifiers;

providing a set of data records associated with an object comprising biological material, the biological material including a plurality of adjacent layers and a feature of interest extending through the plurality of adjacent layers, wherein each of the data records comprises;

two or more slice identifiers identifying, at least, a slice of a first of the plurality of the adjacent layers cut from the object and a slice of a second of the plurality of adjacent layers cut from the object, a delay between cutting the slices of the first and second adjacent layers of the plurality of adjacent layers having been selected from any of hours, days, weeks, months, years, a region of interest in the image of the slice of the second layer having been determined based on a region of interest in the image of the slice of the first layer, a region of interest in the slice of the second layer having been determined based on the region of interest in the image of the slice of the second layer, material from the region of interest having been extracted in the slice of the second layer,

and a process identifier selected from the set of process identifiers, the process identifier indicating a process to which the slice is intended to be subjected; and

providing a user interface for enabling a user to select a data record from the set of data records.

11. The information processing system as set forth in claim 10, wherein the set of process identifiers comprises a process identifier identifying a process selected from the following list of processes related to a slice:

doing nothing with the slice;

archiving the slice;

disposing of the slice;

generating an image of the slice;

containing the slice in a microscope slide or cartridge;

sending the slice to another laboratory;

extracting material from the slice;

freezing the slice;

heating the slice;

sealing the slice;

marking a region of interest on the slice;

staining the slice;

staining the slice by a first staining method;

staining the slice by a second staining method;

defining a color transformation between two staining methods.

12. The information processing system as set forth in claim 10, wherein the set of data records indicates, for at least some of the slices indicated by the slice identifiers, an order corresponding to an order in which the at least some slices were arranged before being cut from the object.

13. The information processing system as set forth in claim 10, wherein the user interface is configured for enabling the user to modify the process identifier in the selected data record, by selecting another process identifier from the set of process identifiers.

14. The information processing system as set forth in claim 10, wherein a first data record in the set of data records comprises a first image or a first image identifier, the first image being a digital image of the slice identified by the slice identifier in the first data record.

15. The information processing system as set forth in claim 14, wherein the first data record comprises information indicating a region of interest in the first image, the region of interest having been defined by a user and the information having being recorded for being transferred to a laboratory in order to prepare a second slice of the object for microdissection.

16. The information processing system as set forth in claim 15, wherein the information processing system is configured for determining a region of interest to be cut from the second slice, by overlaying the first image and a digital second image of the second slice and projecting the region of interest defined in the first image onto the second image.

17. The information processing system as set forth in claim 10, wherein the user interface is configured for enabling the user to add a laboratory identifier selected from a set of laboratory identifiers to the selected data record, and to transmit the selected data record or selected components thereof to a laboratory identified by the selected laboratory identifier.

18. The information processing system as set forth in claim 10, wherein the user interface is configured for enabling the pathologist to ask a laboratory to cut two slices from an object comprising biological material and to generate digital images of the two slices.