Microdissection method and information processing system
First Claim
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1. A method for diagnosing biology, histology, and pathology of an object, the method comprising the acts of:
- providing the object comprising biological material and including a plurality of adjacent layers and a feature of interest extending through the plurality of adjacent layers;
providing an image of a slice of a first of the plurality of the adjacent layers cut from the object;
generating an image of a slice of a second of the plurality of adjacent layers cut from the object, a delay between cutting the slices of the first and second adjacent layers of the plurality of adjacent layers is selected from any of hours, days, weeks, months, years;
determining a region of interest in the image of the slice of the second layer based on a region of interest in the image of the slice of the first layer;
determining a region of interest in the slice of the second layer based on the region of interest in the image of the slice of the second layer; and
extracting material from the region of interest in the slice of the second layer.
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Abstract
A method for use in biology, histology, and pathology includes providing a digital first image of a first slice of an object having biological material; generating a digital second image of a second slice of the object; determining a region of interest in the second image based on a region of interest in the first image; determining a region of interest in the second slice based on the region of interest in the second image; and extracting material from the region of interest in the second slice.
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Citations
18 Claims
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1. A method for diagnosing biology, histology, and pathology of an object, the method comprising the acts of:
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providing the object comprising biological material and including a plurality of adjacent layers and a feature of interest extending through the plurality of adjacent layers; providing an image of a slice of a first of the plurality of the adjacent layers cut from the object; generating an image of a slice of a second of the plurality of adjacent layers cut from the object, a delay between cutting the slices of the first and second adjacent layers of the plurality of adjacent layers is selected from any of hours, days, weeks, months, years; determining a region of interest in the image of the slice of the second layer based on a region of interest in the image of the slice of the first layer; determining a region of interest in the slice of the second layer based on the region of interest in the image of the slice of the second layer; and extracting material from the region of interest in the slice of the second layer. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. An apparatus for use in diagnosing biology, histology, and pathology of an object comprising biological material having a plurality of adjacent layers and a feature of interest extending through the plurality of adjacent layers, comprising:
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a receiver configured to provide an image of a slice of a first of the plurality of the adjacent layers cut from the object; an imager configured to generate an image of a slice of the second layer of a second of the plurality of adjacent layers cut from the object; and a processor configured to; determine a region of interest in the image of the slice of the second layer based on a region of interest in the image of the slice of the first layer, and determine a region of interest in the slice of the second layer based on the region of interest in the image of the slice of the second layer; and an extractor configured to extract material from the region of interest in the slice of the second layer, wherein the imager comprises a microscope objective and the extractor comprises a laser configured to generate a laser beam for transmittal to the slice of the second layer via the microscope objective, wherein the extractor comprises a cutting tool configured to cut material out of the slice of the second layer, and the processor is further configured to; determine, based on the region of interest in the second image, a new position of the cutting tool relative to the slice of the second layer, move the cutting tool relative to the slice of the second layer to the new position, and wherein the slice of the second layer is cut after a delay between cutting the slices of the first and second adjacent layers of the plurality of adjacent layers that is selected from any of hours, days, weeks, months, years.
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9. A non-transitory computer readable medium comprising computer instructions which, when executed by a processor, configure the processor to perform the method for diagnosing biology, histology, and pathology of an object, the method comprising the acts of:
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providing the object comprising biological material and including a plurality of adjacent layers and a feature of interest extending through the plurality of adjacent layers; providing an image of a slice of a first of the plurality of the adjacent layers cut from the object; generating an image of a slice of a second of the plurality of adjacent layers cut from the object, a delay between cutting the slices of the first and second adjacent layers of the plurality of adjacent layers is selected from any of hours, days, weeks, months, years; determining a region of interest in the image of the slice of the second layer based on a region of interest in the image of the slice of the first layer; determining a region of interest in the slice of the second layer based on the region of interest in the image of the slice of the second layer; and extracting material from the region of interest in the slice of the second layer.
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10. An information processing system, for use in biology, histology and pathology, configured for
providing a predefined set of process identifiers; -
providing a set of data records associated with an object comprising biological material, the biological material including a plurality of adjacent layers and a feature of interest extending through the plurality of adjacent layers, wherein each of the data records comprises;
two or more slice identifiers identifying, at least, a slice of a first of the plurality of the adjacent layers cut from the object and a slice of a second of the plurality of adjacent layers cut from the object, a delay between cutting the slices of the first and second adjacent layers of the plurality of adjacent layers having been selected from any of hours, days, weeks, months, years, a region of interest in the image of the slice of the second layer having been determined based on a region of interest in the image of the slice of the first layer, a region of interest in the slice of the second layer having been determined based on the region of interest in the image of the slice of the second layer, material from the region of interest having been extracted in the slice of the second layer,and a process identifier selected from the set of process identifiers, the process identifier indicating a process to which the slice is intended to be subjected; and providing a user interface for enabling a user to select a data record from the set of data records. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18)
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Specification