Lactate sensor
First Claim
1. A lactate sensor comprising an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system,wherein the reagent layer contains lactate oxidase, at least two mediators, and N-(2-acetamide)-2-aminoethanesulfonic acid, andwherein one of the at least two mediators contains a ruthenium compound, and the other mediator of the at least two mediators is a phenazine methosulfate and/or a derivative thereof.
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Abstract
A lactate sensor capable of accurately measuring a lactate concentration in a short period of time. The lactate sensor includes an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system. The reagent layer contains lactate oxidase, it mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid.
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Citations
10 Claims
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1. A lactate sensor comprising an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system,
wherein the reagent layer contains lactate oxidase, at least two mediators, and N-(2-acetamide)-2-aminoethanesulfonic acid, and wherein one of the at least two mediators contains a ruthenium compound, and the other mediator of the at least two mediators is a phenazine methosulfate and/or a derivative thereof.
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7. A method for measuring a lactate concentration, comprising:
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bringing a sample into contact with a reagent layer containing lactate oxidase and N-(2-acetamide)-2-aminoethanesulfonic acid, and electrochemically measuring a reaction between lactate in the sample and the lactate oxidase via at least two mediators, and wherein one of the at least two mediators contains a ruthenium compound, and the other mediator of the at least two mediators is a phenazine methosulfate and/or a derivative thereof. - View Dependent Claims (8, 9)
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10. A method for producing a lactate sensor, the method comprising forming a reagent layer containing lactate oxidase, at least two mediators, and N-(2-acetamide)-2-aminoethanesulfonic acid on an electrode system provided on an insulating substrate, the electrode system including at least a working electrode and a counter electrode, and
wherein one of the at least two mediators contains a ruthenium compound, and one other mediator of the at least two mediators is a phenazine methosulfate and/or a derivative thereof.
Specification