Bioreactors comprising fungal strains

• US 8,999,645 B2
• Filed: 05/06/2011
• Issued: 04/07/2015
• Est. Priority Date: 05/07/2010
• Status: Active Grant

• Current Assignee: Menon Renewable Products, Inc. (Menon & Associates, Inc.)
• Original Assignee: Menon Renewable Products, Inc. (Menon & Associates, Inc.)
• Inventors: Menon, Suresh M., Orchard, Samantha S., Badger, Jessica, Lipson, David, Guidi, Sara, Newman, David
• Primary Examiner(s): Shen, Bin
• Application Number:
  US13/102,965
• Publication Number:
  US 20120058550A1
• Time in Patent Office: 1,432 Days
• Field of Search: 435/6.13
• US Class Current: 435/6.13
• CPC Class Codes: A01N 63/36 Penicillium
• CPC Class Codes: A23K 10/12 by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
• CPC Class Codes: A23K 10/30 from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10
• CPC Class Codes: A23K 20/142 Amino acids; Derivatives thereof
• CPC Class Codes: A23K 20/147 Polymeric derivatives, e.g. peptides or proteins
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 40/20 by moulding, e.g. making cakes or briquettes
• CPC Class Codes: A23K 50/10 for ruminants
• CPC Class Codes: A23K 50/42 Dry feed
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A23K 50/80 for aquatic animals, e.g. fish, crustaceans or molluscs
• CPC Class Codes: A23L 31/00 Edible extracts or preparations of fungi; Preparation or treatment thereof
• CPC Class Codes: A23L 33/10 using additives addition of substantially indigestible substances A23L33/21
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: C11B 1/025 by enzymes or microorganisms, living or dead
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C11C 3/003 by esterification of fatty acids with alcohols C11C3/02 takes precedence
• CPC Class Codes: C12M 21/12 for producing fuels or solvents C12M21/04 takes precedence; liquid carbonaceous fuels C10L1/00, solid fuels C10L5/00
• CPC Class Codes: C12M 25/02 Membranes; Filters filters or filtration in general B01D24/00-B01D41/00
• CPC Class Codes: C12M 27/02 : Stirrer or mobile mixing elements
• CPC Class Codes: C12M 27/20 : Baffles; Ribs; Ribbons; Auger vanes
• CPC Class Codes: C12N 1/14 : Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/145 : Fungal isolates
• CPC Class Codes: C12P 2203/00 : Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source ethanol C12P7/10
• CPC Class Codes: C12P 5/005 : aromatic naphthacene C12P29/00
• CPC Class Codes: C12P 5/02 : acyclic C12P5/007 takes precedence
• CPC Class Codes: C12P 7/6463 : obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/6481 : Phosphoglycerides phosphoglycerides having carboxylic acids with less than seven carbon atoms C12P7/62
• CPC Class Codes: C12R 2001/645 : Fungi ; Processes using fungi
• CPC Class Codes: C12R 2001/80 : Penicillium
• CPC Class Codes: Y02A 40/818 : Alternative feeds for fish, e.g. in aquacultures
• CPC Class Codes: Y10S 426/805 : Pet food for dog, cat, bird, or fish
• CPC Class Codes: Y10S 426/807 : Poultry or ruminant feed

Abstract

Disclosed herein are compositions comprising an isolated cellulose degrading fungus. Also disclosed are culture compositions and bioreactor compositions comprising the cellulose degrading fungus. Further described herein are filtration and extraction devices comprising the cellulose degrading fungus. Still further disclosed are bioprocessing facilities for and methods for producing co-products resulting from one or more bioprocesses of the cellulose degrading fungus.

Claims

1. An admixture for animal feed comprising:

fungal biomass comprising a cellulose degrading fungus, wherein said fungus is a species being the same as NRRL deposit Accession No;

50410, and

animal feed providing additional nutrition,

wherein the wherein the composition comprises 1% to 90% fungal biomass by weight, and wherein the admixture does not contain whey.

2. The admixture of claim 1, wherein the admixture comprises 5% to 20% fungal biomass by weight.

3. The admixture of claim 1, wherein said fungal biomass has been substantially depleted of a lipid product.

4. The admixture of claim 1, wherein the fungal biomass is a product of aerobic fermentation of a feedstock comprising biological matter.

5. The admixture of claim 4, wherein the biological matter comprises at least one of:

simple sugars, cane juice or its condensates or its dry solid evaporate, whey, grains, stover, forage, grasses, oilseed crops, nut shells, nut hulls, fruit pomace, plant waste, algae, wood, wood byproducts, bark, paper, paper products, animal manure, food waste, yard waste, almond hulls, grape pomace, agriculture waste, sawdust.

6. The admixture of claim 4, wherein the biological matter comprises at least one of:

a cellulosic carbon source or a lysate or a hydrolysate thereof, a hemicellulosic carbon source or a lysate or a hydrolysate thereof, and a lignocellulosic carbon source or a lysate or a hydrolysate thereof.

7. The admixture of claim 1, wherein the fungal biomass comprises greater than 25% cell dry weight (cdw) crude protein.

8. The admixture of claim 1, wherein the fungal biomass comprises 5% to 50% of the admixture by weight.

9. The admixture of claim 1, wherein the fungal biomass comprises at least 10% (cdw) lipid comprising triacylglycerol (TAG).

10. The admixture of claim 1, wherein the fungal biomass comprises mycelial residue following extraction of lipids.

11. The admixture of claim 10, wherein the lipids comprise at least one of:

TAG, wax esters, mono-unsaturated hydrocarbon chains, di-unsaturated hydrocarbon chains, and any molecular forms having a straight-chain saturated hydrocarbon portion.

12. The admixture of claim 1, wherein the fungal biomass comprises biomass harvested at a particular time to optimize production of a non-lipid portion of the biomass, the particular time being either a time that an available nitrogen ingredient is exhausted, or a time when log-phase growth terminates.

13. The admixture of claim 1, wherein the fungal biomass comprises 25% to 75% (cdw) crude protein.

14. The admixture of claim 1, wherein the fungal biomass comprises 50% and 90% of the admixture by weight.

15. The admixture of claim 1, wherein the admixture is in the form of a pellet.

16. The admixture of claim 1, wherein the admixture is in the form of a slurry.

17. The admixture of claim 1, wherein the fungal biomass is a dried fungal biomass that is a product of fermentation of a feedstock with said fungus in a liquid culture.

18. The admixture of claim 17, wherein the admixture is produced by a product of combining the dried fungal biomass with said animal feed.

19. The admixture of claim 1, wherein the animal feed is formulated for an aquatic animal.

20. The admixture of claim 1, wherein the animal feed is formulated for a terrestrial animal.