Cysteine engineered antibodies and conjugates
First Claim
1. An isolated cysteine engineered antibody comprising a free cysteine amino acid and a sequence in the heavy chain selected from
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Accused Products
Abstract
Cysteine engineered antibodies comprising a free cysteine amino acid in the heavy chain or light chain are prepared by mutagenizing a nucleic acid sequence of a parent antibody and replacing one or more amino acid residues by cysteine to encode the cysteine engineered antibody; expressing the cysteine engineered antibody; and isolating the cysteine engineered antibody. Certain highly reactive cysteine engineered antibodies were identified by the PHESELECTOR assay. Isolated cysteine engineered antibodies may be covalently attached to a capture label, a detection label, a drug moiety, or a solid support.
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Citations
28 Claims
- 1. An isolated cysteine engineered antibody comprising a free cysteine amino acid and a sequence in the heavy chain selected from
Specification