Controlled electrochemical activation of carbon-based electrodes

• US 9,000,769 B2
• Filed: 10/29/2010
• Issued: 04/07/2015
• Est. Priority Date: 11/23/2009
• Status: Active Grant

• Current Assignee: Proxim Diagnostics, Proxim Diagnostics Corporation
• Original Assignee: Proxim Diagnostics
• Inventors: Briman, Mikhail
• Primary Examiner(s): Koval, Melissa
• Assistant Examiner(s): MILLER, DANIEL R
• Application Number:
  US13/511,143
• Publication Number:
  US 20120242345A1
• Time in Patent Office: 1,621 Days
• Field of Search: None
• US Class Current: 324/446
• CPC Class Codes: G01N 27/26 by investigating electrochemical variables; by using electrolysis or electrophoresis
• CPC Class Codes: G01N 27/30 Electrodes, e.g. test electrodes; Half-cells G01N27/414 takes precedence
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 27/3276 being a hybridisation with immobilised receptors using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68
• CPC Class Codes: G01N 27/38 Cleaning of electrodes

Abstract

Certain embodiments of the present application describe a process to perform activation of carbon based electrodes in a controlled manner, where the degree of activation is monitored and calculated simultaneously with the ongoing activation process. Such an approach is intended to improve the reproducibility of electrodes that may include, but are not limited to, the following parameters: electrochemical capacitance, electrochemical transfer coefficient, amperiometric response to enzymatic reactions.

Claims

1. A process for producing an electrochemical sensor, comprising:

exposing a carbon-based sensor electrode and a reference electrode to an aqueous solution;

cyclically applying an electric potential to the sensor electrode versus the reference electrode, wherein cyclically applying the electric potential comprises;

during a first time period, setting the electric potential to a first potential level at which the carbon-based sensor electrode substantially participates in faradic reactions with electrolytes in the aqueous solution; and

during a second time period after the first time period, setting the electric potential to a second potential level at which the carbon-based sensor electrode does not substantially participate in faradic reactions with electrolytes in the aqueous solution;

measuring an electrochemical capacitance between the carbon-based sensor electrode and the reference electrode during the second time period; and

stopping the cyclical application of the electric potential when the measured electrochemical capacitance reaches a predefined electrochemical capacitance value.

2. The process of claim 1, further comprising introducing functional groups to a surface of the carbon-based sensor electrode through faradic type reactions.

3. The process of claim 2, wherein the process increases electrochemical property reproducibility of the surface of the sensor electrode compared to a process in which the electric potential is applied for a fixed number of cycles.

4. The process of claim 1, wherein the process increases electrochemical property reproducibility of a surface of the sensor electrode compared to a process in which the electric potential is applied for a fixed number of cycles without regard to electrochemical capacitance.

5. An electrochemical sensor produced by the process of claim 1.

6. The electrochemical sensor of claim 5, wherein functional groups are attached to a surface of the carbon-based sensor electrode.

7. The sensor of claim 6, wherein the functional groups comprise at least one of carboxyl and quinone.

8. The electrochemical sensor of claim 7, wherein the electrochemical sensor is an immunological electrochemical biosensor.