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Devices, systems, and methods for microscale isoelectric fractionation

  • US 9,005,417 B1
  • Filed: 10/01/2008
  • Issued: 04/14/2015
  • Est. Priority Date: 10/01/2008
  • Status: Active Grant
First Claim
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1. A microfluidic device comprising:

  • a substrate at least partially defining a fractionation microchannel having at least one cross-sectional dimension equal to or less than 1 mm;

    a first membrane having a first pH disposed in the fractionation microchannel;

    a second membrane having a second pH different from the first pH, the second membrane disposed in the fractionation microchannel a distance from the first membrane;

    an electric field generator configured to apply an electric field perpendicular to the fractionation microchannel to retain analytes within the fractionation microchannel; and

    wherein the fractionation microchannel is configured such that a target analyte having a pH between the first and the second pH is collected in a region of the fractionation channel between the first and second membranes following isoelectric fractionation.

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