Devices, systems, and methods for microscale isoelectric fractionation
First Claim
1. A microfluidic device comprising:
- a substrate at least partially defining a fractionation microchannel having at least one cross-sectional dimension equal to or less than 1 mm;
a first membrane having a first pH disposed in the fractionation microchannel;
a second membrane having a second pH different from the first pH, the second membrane disposed in the fractionation microchannel a distance from the first membrane;
an electric field generator configured to apply an electric field perpendicular to the fractionation microchannel to retain analytes within the fractionation microchannel; and
wherein the fractionation microchannel is configured such that a target analyte having a pH between the first and the second pH is collected in a region of the fractionation channel between the first and second membranes following isoelectric fractionation.
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Abstract
Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.
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Citations
24 Claims
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1. A microfluidic device comprising:
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a substrate at least partially defining a fractionation microchannel having at least one cross-sectional dimension equal to or less than 1 mm; a first membrane having a first pH disposed in the fractionation microchannel; a second membrane having a second pH different from the first pH, the second membrane disposed in the fractionation microchannel a distance from the first membrane; an electric field generator configured to apply an electric field perpendicular to the fractionation microchannel to retain analytes within the fractionation microchannel; and wherein the fractionation microchannel is configured such that a target analyte having a pH between the first and the second pH is collected in a region of the fractionation channel between the first and second membranes following isoelectric fractionation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A microfluidic system comprising:
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a substrate; a preparation module including a fractionation microchannel defined in part by the substrate, wherein the fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm, the preparation module further comprising; a plurality of membranes disposed in the fractionation microchannel, including a first and second membrane, the first membrane having a first pH and the second membrane having a second pH different from the first pH, the second membrane positioned a distance from the first membrane; the fractionation microchannel configured such that a target analyte having a pH between the first and the second pH is collected in a region of the fractionation channel between the first and second membranes following isoelectric fractionation; and a first analyte collection channel in fluid communication with the fractionation microchannel between the first and second membranes, the first analyte collection channel configured to receive the target analyte following fractionation; a transport module supported by the substrate, the transport module including a transport channel in fluid communication with the first analyte collection channel; an analysis module supported by the substrate, the analysis module configured to receive the target analyte from the transport module and analyze the target analyte; and an electric field generator configured to apply an electric field perpendicular to the fractionation microchannel to retain analytes within the fractionation microchannel. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification