Method and biochip for studying a chemical sample

• US 9,005,951 B2
• Filed: 12/12/2006
• Issued: 04/14/2015
• Est. Priority Date: 12/13/2005
• Status: Active Grant

• Current Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Inventors: Abraham-Fuchs, Klaus
• Primary Examiner(s): CROW, ROBERT THOMAS
• Application Number:
  US11/637,027
• Publication Number:
  US 20070141553A1
• Time in Patent Office: 3,045 Days
• Field of Search: None
• US Class Current: 435/243
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00

Abstract

A method for studying a biological sample in a biochip (“lab-on-a-chip”) is disclosed. In at least one embodiment of the method, a biological sample is introduced into the biochip, the sample is subjected to at least one preparation step and, at the end of a measurement cycle, the concentration of a particular analyte is measured. The concentration of a marker substance, which for example is a reaction substance involved in a preparation step, is furthermore measured. A corresponding biochip is further disclosed.

Claims

1. A method for studying a biological sample in a biochip having a closed unit configuration with a system of microfluidic channels, a sample input and a reservoir that contains a reaction substance that is covered by a disintegrateable protective layer, comprising:

introducing a biological sample into the biochip;

causing the protective layer to disintegrate so as to result in the reaction substance contained in the reservoir becoming mixed with the sample,

subjecting the biological sample to at least one preparation step in which the reaction substance is involved;

measuring, at the end of a measurement cycle, at least one of a concentration and a presence of a particular analyte in the prepared biological sample; and

measuring at least one of a concentration and a presence of a marker substance,

wherein the marker substance is a breakdown product of the protective membrane, wherein the measurement of the at least one of the concentration and the presence of the marker substance is used for at least one of controlling the measurement cycle and quality control

wherein, prior to said preparation and measuring steps, a first sensor measures the concentration of a marker substance to detect whether the content of the reservoir has been properly brought into contact with the sample, and depending on the concentration of the marker substance detected, the measurement cycle may be terminated, the step of causing the protective layer to disintegrate repeated, or results of said measuring steps subsequently corrected.

2. The method as claimed in claim 1, wherein the marker substance is the reaction substance involved in the preparation step.

3. The method as claimed in claim 1, wherein the marker substance is stored in a dried form on the biochip before the measurement cycle.

4. The method as claimed in claim 1, wherein the marker substance is an enzyme which is involved in the preparation step, and which is used for cell lysis.

5. The method as claimed in claim 1, wherein a plurality of different protective membranes are on the biochip and the plurality of different protective membranes consist of different chemical compositions in order that disintegration of the plurality of different protective membranes is detected individually.

6. The method as claimed in claim 1, wherein the at least one of the concentration and the presence of the marker substance is measured after a particular step during the measurement cycle.

7. The method as claimed in claim 1, wherein the at least one of the concentration and the presence of the marker substance is measured several times during the measurement cycle.

8. The method as claimed in claim 2, wherein the marker substance is stored in a dried form on the biochip before the measurement cycle.

9. The method as claimed in claim 1, wherein the marker substance is a protein.