Functionalized membrane for a chamber for encapsulating cells producing at least one substance of therapeutic interest and bioartificial organ comprising such a membrane

• US 9,005,960 B2
• Filed: 05/31/2011
• Issued: 04/14/2015
• Est. Priority Date: 06/04/2010
• Status: Expired due to Fees

• Current Assignee: Association Pour Les Transferts De Technologies Du Mans, Rotalactis SRL
• Original Assignee: Association Pour Les Transferts De Technologies Du Mans, Rotalactis SRL
• Inventors: Legeay, Gilbert, Sigrist, Sverine, Coudreuse, Arnaud
• Primary Examiner(s): Sweet, Thomas J
• Assistant Examiner(s): Diop, Rokhaya
• Application Number:
  US13/701,750
• Publication Number:
  US 20130131828A1
• Time in Patent Office: 1,414 Days
• Field of Search: 623/64, 623/71, 623/72, 623/73, 623/74, 623/75, 424/422, 424/424, 424/473, 435/295.3
• US Class Current: 435/297.1
• CPC Class Codes: A61F 2/02 Prostheses implantable into the body closure means for urethra or rectum or for artificial body openings therefor A61F2/0004
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 33/0005 Use of materials characterised by their function or physical properties
• CPC Class Codes: A61P 1/18 for pancreatic disorders, e.g. pancreatic enzymes
• CPC Class Codes: B01D 67/0088 Physical treatment with compounds, e.g. swelling, coating or impregnation
• CPC Class Codes: B01D 67/009 with wave-energy, particle-radiation or plasma
• CPC Class Codes: B01D 67/0093 Chemical modification
• CPC Class Codes: B01D 69/105 Support pretreatment
• CPC Class Codes: B01D 69/1216 Three or more layers
• CPC Class Codes: B01D 69/144 containing embedded or bound biomolecules

Abstract

The present invention relates to a functionalized semi-permeable membrane composed of a porous biocompatible support pretreated so as to increase the surface energy thereof and characterized in that it includes at least two layers, each having a hydrophilic polymer and at least one biological active molecule, and to the use thereof in particular for producing a bioartificial organ.

Claims

1. A functionalized semi-permeable membrane for production of a chamber for encapsulating secretory cells of a bioartificial organ composed of a porous biocompatible support, wherein:

said porous biocompatible support is pretreated so that its surface energy is greater than or equal to 50 mJ·

m<sup>−

2</sup>;

pores of said porous biocompatible support have an inner size between 5 and 100 nm, and

said functionalized semi-permeable membrane comprises at least two layers each comprising a hydrophilic polymer and at least one biologically active molecule.

2. The functionalized semi-permeable membrane as claimed in claim 1, wherein the hydrophilic polymer is chosen from cellulose compounds, polyacrylamides and their copolymers, polyvinylpyrrolidone (PVP) and its copolymers, polyvinyl alcohols, copolymers of vinyl acetate, polyethylene glycols, polypropylene glycols, hydrophilic poly(meth)acrylates, polysaccharides, hyaluronic acid-based polymers and chitosans.

3. The functionalized semi-permeable membrane as claimed in claim 1, wherein the biologically active molecule is chosen from anti-inflammatory agents, anti-infective agents, anesthetics, growth factors, angiogenesis-stimulating and/or vascularization-inducing agents, wound-healing agents, immunosuppressive agents, antithrombotic agents including antiaggregation agents and anticoagulating agents, inhibitors of angiotensin-converting enzyme (ACE), or molecules stimulating insulin secretion.

4. The functionalized semi-permeable membrane as claimed in claim 1, wherein the functionalized semi-permeable membrane comprises at least two layers of hydrophilic polymer, each layer comprising at least one biologically active molecule, a first of said layers is placed between said support and a second layer and comprises a cell growth factor and the second of said layers comprises an antithrombotic agent.

5. The functionalized semi-permeable membrane as claimed in claim 4, wherein said cell growth factor is VEGF and in that said antithrombotic agent is heparin.

6. A chamber for encapsulating secretory cells producing at least one substance of therapeutic interest, characterized in that its walls consist of a functionalized semi-permeable membrane as claimed in claim 1 delimiting a space capable of containing the secretory cells producing at least one substance of therapeutic interest.

7. A bioartificial organ, wherein the organ comprises at least one encapsulation chamber as claimed in claim 6.

8. The bioartificial organ as claimed in claim 7, wherein the organ is a bioartificial pancreas containing cells of the pancreatic islets.

9. The functionalized semi-permeable membrane as claimed in claim 2, wherein the biologically active molecule is chosen from anti-inflammatory agents, anti-infective agents, anesthetics, growth factors, angiogenesis-stimulating andor vascularization-inducing agents, wound-healing agents, immunosuppressive agents, antithrombotic agents including antiaggregation agents and anticoagulating agents, inhibitors of angiotensin-converting enzyme (ACE), or molecules stimulating insulin secretion.