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Stem cells culture systems

  • US 9,005,965 B2
  • Filed: 01/13/2011
  • Issued: 04/14/2015
  • Est. Priority Date: 12/29/2004
  • Status: Active Grant
First Claim
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1. A method for maintaining human pluripotent stem cells in an undifferentiated state, comprising:

  • providing human umbilical cord fibroblast feeder cells isolated and expanded from human umbilical cord;

    incubating the human pluripotent stem cells in a culture system comprising the human umbilical fibroblast feeder cells, the feeder cells consisting essentially of human umbilical cord fibroblast feeder cells, the culture system being free from antibacterial agents and mercaptoethanol; and

    maintaining the human pluripotent stem cells in an undifferentiated state for at least 10 weeks.

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