Stem cells culture systems
First Claim
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1. A method for maintaining human pluripotent stem cells in an undifferentiated state, comprising:
- providing human umbilical cord fibroblast feeder cells isolated and expanded from human umbilical cord;
incubating the human pluripotent stem cells in a culture system comprising the human umbilical fibroblast feeder cells, the feeder cells consisting essentially of human umbilical cord fibroblast feeder cells, the culture system being free from antibacterial agents and mercaptoethanol; and
maintaining the human pluripotent stem cells in an undifferentiated state for at least 10 weeks.
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Abstract
Provided are systems and methods for providing human cell cultures. Further provided are cultures of feeder cells for use in stem cell technology, as well as cultures, culture systems and methods for maintenance and propagating of stem cells in an undifferentiated state as well as for the development of somatic cells cultures from stem cells, the somatic cell cultures being free of extraembryonic cells.
15 Citations
9 Claims
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1. A method for maintaining human pluripotent stem cells in an undifferentiated state, comprising:
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providing human umbilical cord fibroblast feeder cells isolated and expanded from human umbilical cord; incubating the human pluripotent stem cells in a culture system comprising the human umbilical fibroblast feeder cells, the feeder cells consisting essentially of human umbilical cord fibroblast feeder cells, the culture system being free from antibacterial agents and mercaptoethanol; and maintaining the human pluripotent stem cells in an undifferentiated state for at least 10 weeks. - View Dependent Claims (2, 3, 4, 5)
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6. A method for maintaining human pluripotent stem cells in an undifferentiated state, comprising:
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providing human umbilical cord fibroblast feeder cells comprising expanding human umbilical cord cells to obtain human umbilical cord feeder cells, and isolating human umbilical cord fibroblast feeder cells from the human umbilical cord feeder cells, to obtain human umbilical cord fibroblast feeder cells; and incubating the human pluripotent stem cells in a culture system comprising the human umbilical cord fibroblast feeder cells, and the culture system is free from antibacterial agents and mercaptoethanol. - View Dependent Claims (7, 8, 9)
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Specification