Determining feline proBNP

• US 9,005,984 B2
• Filed: 02/27/2009
• Issued: 04/14/2015
• Est. Priority Date: 09/08/2004
• Status: Active Grant

• Current Assignee: The Antibody Lab GmbH
• Original Assignee: Biomedica Medizinprodukte GmbH & Co., KG
• Inventors: Woloszczuk, Wolfgang, Hawa, Gerhard
• Primary Examiner(s): FOSTER, CHRISTINE E
• Application Number:
  US12/394,637
• Publication Number:
  US 20090170136A1
• Time in Patent Office: 2,237 Days
• Field of Search: None
• US Class Current: 436/86
• CPC Class Codes: C07K 16/26 against hormones ; against hormone releasing or inhibiting factors
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
• CPC Class Codes: G01N 2333/58 Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Brain natriuretic peptide [BNP, proBNP]; Cardionatrin; Cardiodilatin
• CPC Class Codes: G01N 2800/32 Cardiovascular disorders
• CPC Class Codes: G01N 33/68 involving proteins, peptides or amino acids involving lipoproteins G01N33/92

Abstract

Methods and compositions are disclosed for determining feline proBNP or fragments thereof in a sample. In one method, feline proBNP or fragments thereof are determined by providing a feline sample, contacting the sample with at least one antibody that binds an epitope in the region from amino acids 68 to 80 of feline proBNP, and determining the presence of the feline proBNP or fragments thereof present in the sample. Antibodies that bind feline proBNP and kits comprising such antibodies are also disclosed.

Claims

1. A method for detecting feline proBNP or fragments thereof, the method comprising:

obtaining a blood sample from a feline subject;

contacting said blood sample with a first antibody raised against peptide sequence VLAPHDNVLRALRRLGSSKM (SEQ ID;

     9) and a second antibody raised against peptide sequence MALGPLQQGHS (SEQ ID;

     7); and

detecting binding of the first antibody and the second antibody, thereby detecting feline proBNP or fragments thereof in the blood sample.

2. The method of claim 1, wherein said step of detecting binding is performed by radioimmunoassay.

3. The method of claim 1, wherein said step of detecting binding is performed by enzyme immunoassay.

4. The method of claim 1, wherein said first antibody or said second antibody is bound to a solid phase.