Apparatus for and method of automated processing of biological samples

• US 9,011,772 B2
• Filed: 05/11/2010
• Issued: 04/21/2015
• Est. Priority Date: 05/12/2009
• Status: Active Grant

• Current Assignee: Life Technologies AS (Perspecta, Inc.)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Norderhaug, Lars, Egeberg, Morten, Nokleby, Lars, Kahatt, Espir, Hogan, Jamie, Zgonc, Kornelija, Trolson, Norman, Rivrud, Tommy
• Primary Examiner(s): WRIGHT, PATRICIA KATHRYN
• Application Number:
  US13/147,084
• Publication Number:
  US 20110287447A1
• Time in Patent Office: 1,806 Days
• US Class Current: 422/63
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/0275 Interchangeable or disposable dispensing tips
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/563 Joints or fittings in general F16L; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
• CPC Class Codes: B01L 9/543 for disposable pipette tips, e.g. racks or cassettes
• CPC Class Codes: G01N 2035/00227 Monitoring a process (online)
• CPC Class Codes: G01N 2035/00237 Handling microquantities of analyte, e.g. microvalves, capillary networks
• CPC Class Codes: G01N 2035/0436 with pre-packaged reagents, i.e. test-packs
• CPC Class Codes: G01N 2035/0498 Drawers used as storage or dispensing means for vessels or cuvettes
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 2035/1039 Micropipettes, e.g. microcapillary tubes
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

Provided herein is a bioprocessing device, bioprocessing card, and fluidics cartridge for performing automated bioprocessing of a sample. The bioprocessing card may include a plurality of pipette tips; and at least one pump in fluid communication with the plurality of pipette tips. In some embodiments, the pumps and the pipette tips are in fluid communication through a processing channel which may be a microscale channel. Also provided herein is an automated bioprocessing device comprising: at least one bioprocessing card; at least one fluidic cartridge; and an automated control system configured to control automated bioprocessing of a sample. Further provided herein are methods of use of the device, card, and cartridge.

Claims

1. A system for sample bioprocessing, comprising:

a. a fluidics cartridge that includes a first well and a second well, wherein the first well holds a fluid sample that includes one or more magnetic particles;

b. a bioprocessing card that includes a first pipette tip, a second pipette tip, and a pump that are in fluid communication along a processing channel and that is placed in proximity to the fluidics cartridge so that the first pipette tip is in fluid communication with the first well and the second pipette tip is in fluid communication with the second well wherein the processing channel is a mesoscale channel or a microscale channel, the first pipette tip and second pipette tip include individual valves;

c. a manifold that is in physical communication with the bioprocessing card;

d. a magnetic assembly including at least one electromagnet or permanent magnet that is placed in proximity to the fluidics cartridge;

e. a memory that includes instructions for a sample preparation protocol; and

f. a processor that is in electronic communication with the manifold, the magnetic assembly, and the memory, that reads the instructions from the memory, and that according to the instructions

i. activates the magnetic assembly to apply a magnetic field to the first well so that at least one magnetic particle of the one or more magnetic particles of the fluid sample is attracted and

ii. signals the manifold to apply pressure and/or vacuum to the bioprocessing card activating the fluid valves of the first pipette tip, the second pipette tip, and the pump to move a portion of the fluid sample from the first well though the processing channel, to the second well producing a bioprocessed sample in the first well;

g. a housing containing the fluidics cartridge, the bioprocessing card, the manifold, the magnetic assembly, the memory and the processor, wherein at least one vent is located on a portion of the housing configured to provide heat dispersion.

2. The system of claim 1, further comprising a heat/cooling block that is placed in proximity to the fluidics cartridge, that is in electronic communication with the processor, and that receives a signal from the processor to heat or cool of the fluidics cartridge according to the instructions.

3. The system of claim 1, further comprising an input/output device from which the processor can also read the instructions, wherein the input/output device is in electronic communication with the processor.

4. The system of claim 3, further comprising a user interface device from which the processor receives a protocol selection from a user, wherein the input/output device is in electronic communication with the processor.

5. The system of claim 4, wherein the processor receives a protocol parameter from the user interface device that indicates a parameter selected by the user.

6. The system of claim 4 or 5, wherein the processor sends a system status to the user interface device.

7. The system of claim 1, wherein the instructions comprise a scripting language format.

8. The system of claim 7, wherein the scripting language format comprises a bioprocessing sequence.

9. The system of claim 8, wherein the bioprocessing sequence comprises a step.

10. The system of claim 9, wherein the processor translates the step into a processing device signal.

11. The system of claim 1, wherein the manifold applies pressure and/or vacuum to the bioprocessing card by activating one or more manifold valves of a plurality of manifold valves on the manifold.

12. The system of claim 11, wherein the manifold activates the one or more manifold valves using a matrix of address lines to allow more than one bioprocessing card to perform the sample preparation protocol at substantially the same time.

13. The system of claim 12, wherein each manifold valve of the plurality of manifold valves can be addressed using two address lines of the matrix of address lines.

14. The system of claim 1, wherein the bioprocess card comprises:

a plurality of pipette tips in addition to the first and second pipette tip;

at least one pump in fluid communication with the plurality of pipette tips; and

a plurality of valves, wherein the plurality of pipette tips and the at least one pump are in fluid communication through the processing channel.

15. The system of claim 14, wherein the bioprocess card further comprises a plurality of control fluid connectors, wherein the control fluid connector is configured to be in communication with the pipette tips and wherein a control fluid connector is configured to be in communication with the at least one pump.

16. The system of claim 15, wherein the bioprocessing card further comprising a membrane.

17. The system of claim 16, wherein the bioprocessing card further comprises a sealing foil.

18. The system of claim 1, wherein the fluidics cartridge includes an opening configured to receive a container configured to contain and confine a liquid.

19. A method for sample bioprocessing, comprising:

a. reading instructions for a sample preparation protocol from a memory using a processor;

b. activating a magnetic assembly to apply a magnetic field to a first well of a fluidics cartridge so that at least one magnetic particle of one or more magnetic particles of a fluid sample is attracted using the processor, wherein the fluidics cartridge includes the first well and a second well, wherein the first well holds the fluid sample that includes the one or more magnetic particles, and wherein the magnetic assembly includes at least one electromagnet or permanent magnet that is placed in proximity to the fluidics cartridge; and

c. signaling a manifold to apply pressure and/or vacuum to a bioprocessing card activating fluid valves of a first pipette tip, a second pipette tip, and a pump to move a portion of the fluid sample from the first well through a processing channel wherein the processing channel is a mesoscale channel or a microscale channel, to the second well producing a bioprocessed sample in the first well using the processor, wherein the bioprocessing card includes the first pipette tip, the second pipette tip, and the pump that are in fluid communication along the processing channel, wherein the bioprocessing card is in proximity to the fluidics cartridge so that the first pipette tip is in fluid communication with the first well and the second pipette tip is in fluid communication with the second well, and wherein the manifold is in physical communication with the bioprocessing card

d. dispersing heat generated from executing the sample preparation protocol through at least one vent is in a housing, the housing containing the fluidics cartridge, the bioprocessing card, the manifold, the magnetic assembly, the memory and the processor.