Method and apparatus for separating biological materials
First Claim
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1. A separation system to separate a multiple component material into at least two fractions, comprising:
- a separation container defining a volume within an interior wall, a first end wall, and a second end wall;
a buoy separation system in the volume operable to move to a selected interface of the multiple component material when the multiple component material is separated, the buoy separation system including;
a first buoy portion having a passage between a first side and a second side of the first buoy portion;
a second buoy portion at a selected distance from the second side of the first buoy portion, wherein the selected distance defines a collection volume between the second side of the first buoy portion and the second buoy portion;
a third buoy portion extending between the first buoy portion and the second buoy portion;
a valve assembly extending from the third buoy portion, the valve assembly has a sealing member the is formed separate and distinct and is moveable relative to a flexible member, wherein the flexible member is positioned between the sealing member and the second buoy portion and holds the sealing member against the first buoy portion to seal the passage in the first buoy portion to separate the collection volume from a volume exterior to the collection volume on the first side of the first buoy portion;
wherein the valve assembly is operable to be unsealed from the first buoy portion to allow a selected fraction to move into the collection volume.
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Abstract
According to various embodiments, a system can be provided to separate undifferentiated cells and/or stromal cells from a whole tissue sample. The whole tissue sample can be any appropriate tissue sample obtained directly from a patient. The tissue sample can be obtained during a selected operating procedure for immediate or quick application or re-application to the patient. Accordingly, autologous cells can be obtained intraoperatively for application to a patient substantially soon after obtaining a whole tissue sample.
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Citations
25 Claims
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1. A separation system to separate a multiple component material into at least two fractions, comprising:
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a separation container defining a volume within an interior wall, a first end wall, and a second end wall; a buoy separation system in the volume operable to move to a selected interface of the multiple component material when the multiple component material is separated, the buoy separation system including; a first buoy portion having a passage between a first side and a second side of the first buoy portion; a second buoy portion at a selected distance from the second side of the first buoy portion, wherein the selected distance defines a collection volume between the second side of the first buoy portion and the second buoy portion; a third buoy portion extending between the first buoy portion and the second buoy portion; a valve assembly extending from the third buoy portion, the valve assembly has a sealing member the is formed separate and distinct and is moveable relative to a flexible member, wherein the flexible member is positioned between the sealing member and the second buoy portion and holds the sealing member against the first buoy portion to seal the passage in the first buoy portion to separate the collection volume from a volume exterior to the collection volume on the first side of the first buoy portion; wherein the valve assembly is operable to be unsealed from the first buoy portion to allow a selected fraction to move into the collection volume. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 22, 23, 24)
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10. A separation system including a buoy separation system to separate a multiple component material into at least a first fraction, the buoy separation system comprising:
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a first buoy portion having a passage between a first side and a second side of the first buoy portion; a second buoy portion spaced a selected distance from the second side of the first buoy portion, wherein the selected distance defines a collection volume between the second side of the first buoy portion and the second buoy portion; and a valve assembly having a sealing member and a flexible member, the flexible member having a central portion fixed relative to the first buoy portion and extending a dimension from the central portion to an outer edge great enough to extend substantially the entire selected distance between the first buoy portion and the second buoy portion when at least the central portion is fixed relative to the first buoy portion; wherein the flexible member holds the sealing member against the first buoy portion to seal the passage in the first buoy portion thus maintaining a separation of the collection volume from a volume exterior on the first side of the first buoy portion; wherein the valve assembly is operable to be unsealed from the first buoy portion to allow the first fraction to move into the collection volume. - View Dependent Claims (11, 12, 13, 14, 15, 25)
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16. A separation system including a buoy separation system to separate a multiple component material into at least a first fraction, the buoy separation system comprising:
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a first buoy portion having a passage between a first side and a second side of the first buoy portion; a second buoy portion positioned at a selected distance from the second side of the first buoy portion, wherein the selected distance defines a collection volume between the second side of the first buoy portion and the second buoy portion; a third buoy portion extending between the first buoy portion and the second buoy portion; and a valve assembly having a sealing member distinct and separate from a flexible member; wherein the flexible member is between the sealing member and the second buoy portion and contacts the sealing member to engage the sealing member against the first buoy portion to seal the passage in the first buoy portion thus maintaining a separation of the collection volume from a volume exterior on the first side of the first buoy portion; wherein the valve assembly is operable to be unsealed from the first buoy portion. - View Dependent Claims (17, 18, 19, 20, 21)
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Specification