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Recombinant carrier molecule for expression, delivery and purification of target polypeptides

  • US 9,012,199 B2
  • Filed: 10/23/2013
  • Issued: 04/21/2015
  • Est. Priority Date: 05/22/2003
  • Status: Active Grant
First Claim
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1. A nucleic acid that encodes a carrier protein comprising a recombinant carrier molecule, wherein the recombinant carrier molecule has at least 90% amino acid identity to an amino acid sequence of a modified lichenase B (licB) polypeptide that is based on a rearranged amino acid sequence of the Clostridium thermocellum wild-type licB (wild-type licB) amino acid sequence encoded by the nucleic acid sequence set forth in SEQ ID NO:

  • 18, wherein the modified licB polypeptide comprises original N-terminal and C-terminal regions that correspond in sequence to N- and C-terminal polypeptides obtained by splitting the amino acid sequence encoded by SEQ ID NO;

    18 within the loop region defined by amino acid residues 82-94 of the amino acid sequence encoded by SEQ ID NO;

    18, wherein the modified licB polypeptide comprises the original C-terminal region fused upstream of the original N-terminal region, and wherein the recombinant carrier molecule optionally has a heterologous polypeptide comprising a therapeutic polypeptide or a disease-associated epitope fused thereto.

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