Controlled release of growth factors and signaling molecules for promoting angiogenesis
First Claim
1. A method of inducing growth of new blood vessels either from existing blood vessels, or creation of de novo vessels, or a combination thereof, in a target tissue of a mammal, comprising contacting a mammalian cell by injecting into a tissue a device comprising an alginate hydrogel scaffold composition comprising N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester (DAPT) and vascular endothelial growth factor (VEGF), wherein said scaffold composition temporally controls release of the DAPT and the VEGF, wherein the VEGF said bioactive composition induces angiogenesis, arteriogenesis, or vasculogenesis, and wherein the VEGF comprises VEGF121, VEGF145, VEGF165, VEGF189, or VEGF206.
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Abstract
The present invention comprises compositions, methods, and devices for delivering angiogenic factors and signaling molecules to a target tissue, and controlling the release of these factors and signaling molecules to spatially and temporally restrict their release and dissemination, for the purpose of promoting angiogenesis in target tissues.
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Citations
29 Claims
- 1. A method of inducing growth of new blood vessels either from existing blood vessels, or creation of de novo vessels, or a combination thereof, in a target tissue of a mammal, comprising contacting a mammalian cell by injecting into a tissue a device comprising an alginate hydrogel scaffold composition comprising N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester (DAPT) and vascular endothelial growth factor (VEGF), wherein said scaffold composition temporally controls release of the DAPT and the VEGF, wherein the VEGF said bioactive composition induces angiogenesis, arteriogenesis, or vasculogenesis, and wherein the VEGF comprises VEGF121, VEGF145, VEGF165, VEGF189, or VEGF206.
- 15. A method of inducing growth of new blood vessels either from existing blood vessels, or creation of de novo vessels, or a combination thereof, in a target tissue of a mammal, comprising contacting a mammalian cell by injecting into a tissue a device comprising an alginate hydrogel scaffold composition comprising N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester (DAPT) and a bioactive composition selected from the group consisting of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF), wherein said scaffold composition temporally controls release of the DAPT and the bioactive composition, wherein the bioactive composition induces angiogenesis, arteriogenesis, or vasculogenesis, and wherein the VEGF comprises VEGF121, VEGF145, VEGF165, VEGF189, or VEGF206.
- 24. A method of inducing growth of new blood vessels either from existing blood vessels, or creation of de novo vessels, or a combination thereof, in a target tissue of a mammal, comprising contacting a mammalian cell by injecting into a tissue a device comprising a hydrogel scaffold composition comprising N-[N-(3,5-Difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester (DAPT), vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF), wherein said scaffold composition temporally controls release of the DAPT, VEGF, and PDGF, and wherein the VEGF and PDGF induce angiogenesis, arteriogenesis, or vasculogenesis.
Specification