Flow cytometer apparatus for three dimensional difraction imaging and related methods
First Claim
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1. A flow cytometer assembly, comprising:
- a fluid controller configured to form a hydrodynamically focused flow stream, the stream including an outer sheath fluid and an inner core fluid;
a coherent light source configured to illuminate a particle in the inner core fluid;
a detector configured to detect a spatially coherent distribution of elastically scattered light from the particle excited by the coherent light source; and
an analyzing module configured to extract a three-dimensional morphology parameter of the particle from a spatially coherent distribution of the elastically scattered light.
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Abstract
A flow cytometer assembly includes a fluid controller configured to form a hydrodynamically focused flow stream including an outer sheath fluid and an inner core fluid. A coherent light source is configured to illuminate a particle in the inner core fluid. A detector is configured to detect a spatially coherent distribution of elastically scattered light from the particle excited by the coherent light source. An analyzing module configured to extract a three-dimensional morphology parameter of the particle from a spatially coherent distribution of the elastically scattered light.
34 Citations
37 Claims
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1. A flow cytometer assembly, comprising:
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a fluid controller configured to form a hydrodynamically focused flow stream, the stream including an outer sheath fluid and an inner core fluid; a coherent light source configured to illuminate a particle in the inner core fluid; a detector configured to detect a spatially coherent distribution of elastically scattered light from the particle excited by the coherent light source; and an analyzing module configured to extract a three-dimensional morphology parameter of the particle from a spatially coherent distribution of the elastically scattered light. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of analyzing particles in a flow cytometer to determine three-dimensional morphology parameters, the method comprising:
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forming a hydrodynamically focused flow stream, the stream including an outer sheath fluid and an inner core fluid; illuminating a particle in the inner core fluid with a coherent light source; detecting elastically scattered light from the particle excited by the coherent light source; and extracting a three-dimensional morphology parameter of the particle from a spatially coherent distribution of the elastically scattered light. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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31. A computer program product for analyzing particles in a flow cytometer to determine three-dimensional morphology parameters, the flow cytometer having a hydrodynamically focused flow stream including an outer sheath fluid and an inner core fluid, a coherent light source configured to illuminate a particle, and a detector for detecting a coherent distribution of elastically scattered light from the particle excited by the coherent light source, the computer program product comprising a computer usable storage medium having computer-readable program code embodied in the medium, the computer-readable program code comprising:
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computer-readable program code that is configured to receive diffraction image data comprising a spatially coherent distribution of elastically scattered light from the flow cytometer; and computer-readable program code that is configured to extract a three-dimensional morphology parameter of the particle from the spatially coherent distribution of the elastically scattered light. - View Dependent Claims (32, 33, 34, 35, 36, 37)
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Specification