×

Assays and other reactions involving droplets

  • US 9,017,948 B2
  • Filed: 02/04/2014
  • Issued: 04/28/2015
  • Est. Priority Date: 03/07/2007
  • Status: Active Grant
First Claim
Patent Images

1. A method of amplification comprising:

  • a. generating a first plurality of droplets comprising polynucleotide primers, wherein the first plurality of droplets excludes polynucleotides to be amplified;

    b. generating a second plurality of droplets comprising polynucleotides to be amplified, wherein the polynucleotides are obtained from a whole genome;

    c. merging first droplets of the first plurality of droplets with second droplets of the second plurality of droplets to form coalesced droplets; and

    d. performing amplification reactions on the polynucleotides in the coalesced droplets to form amplicons.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×