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Methods and compositions for tagging and identifying polynucleotides

  • US 9,018,365 B2
  • Filed: 03/28/2013
  • Issued: 04/28/2015
  • Est. Priority Date: 02/10/2005
  • Status: Active Grant
First Claim
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1. A composition of nucleic acid molecules made by the following method:

  • ligating a discriminatory oligonucleotide tag to fragmented genomic DNA so that substantially every target polynucleotide in said fragmented genomic DNA receives a tag that comprises a unique nucleotide sequence, to produce a tagged sample; and

    amplifying the target polynucleotides in said tagged sample by polymerase chain reaction to produce a plurality of amplicons that each comprise a unique tag.

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