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Microarray synthesis and assembly of gene-length polynucleotides

  • US 9,023,601 B2
  • Filed: 09/30/2011
  • Issued: 05/05/2015
  • Est. Priority Date: 09/12/2002
  • Status: Expired due to Term
First Claim
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1. A composition for assembly of a target polynucleotide sequence, the composition comprising a plurality of different synthesized oligonucleotide sequences, wherein the plurality of different synthesized oligonucleotide sequences are immobilized on a solid or porous surface, each oligonucleotide sequence comprising a fragment of a target polynucleotide sequence and at least one flanking sequence, wherein the at least one flanking sequence is at the 3′

  • end, or the 5′

    end, or the 3′

    end and the 5′

    end of each fragment, and wherein each flanking sequence comprises a primer binding site and a sequence segment designed to permit removal of the primer binding site such that the fragments together comprise the target polynucleotide sequence.

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