Oil producing microbes and method of modification thereof

• US 9,023,616 B2
• Filed: 07/30/2007
• Issued: 05/05/2015
• Est. Priority Date: 08/01/2006
• Status: Active Grant

• Current Assignee: DSM Nutritional Products AG (DSM-Firmenich AG)
• Original Assignee: DSM Nutritional Products AG (DSM-Firmenich AG)
• Inventors: Radianingtyas, Helia, Burja, Adam M., Barrow, James Colin
• Primary Examiner(s): BURKHART, MICHAEL D
• Application Number:
  US12/309,895
• Publication Number:
  US 20100285105A1
• Time in Patent Office: 2,836 Days
• Field of Search: None
• US Class Current: 435/41
• CPC Class Codes: A61P 3/02 Nutrients, e.g. vitamins, minerals
• CPC Class Codes: A61P 3/04 Anorexiants; Antiobesity agents
• CPC Class Codes: A61P 3/06 Antihyperlipidemics
• CPC Class Codes: A61P 3/08 for glucose homeostasis pancreatic hormones A61P5/48
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 39/06 Free radical scavengers or antioxidants
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 5/50 for increasing or potentiating the activity of insulin
• CPC Class Codes: C12P 7/6463 obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/6472 containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Abstract

Disclosed are compositions and methods related to a eukaryote of the order Thraustochytriales and family Thraustochytriaceae which when cultured produce quantities of unsaturated fatty acids, such as omega 3 (n-3) and/or omega 6 (n-6) oils, such as DHA, EPA and DPA, capable of being purified and used as all such compositions are used and more, because of their means of production.

Claims

1. A method of preparing a lipid composition, the method comprising÷

culturing an oil producing microbe in a heterotrophic medium comprising a fatty acid production antagonist, wherein the oil producing microbe has an 18S sequence having at least 97% identity to the sequence set forth in SEQ ID NO;

1.

2. The method of claim 1, further comprising isolating the lipid composition.

3. The method of claim 1, wherein the ratio of omega-3 and omega-6 fatty acids to omega-9 fatty acids is increased.

4. The method of claim 3, wherein the overall fatty acid content is unaltered.

5. The method of claim 1, wherein the oil producing microbe is a diatom, a microalgae, a fungi, a bacterium, or a protist.

6. The method of claim 1, wherein the oil producing microbe is selected from the group consisting of the order Thraustochytriales, the family Thraustochytriaceae, the genus Thraustochytrium, the genus Schizochytrium, Thraustochytrium sp., Thraustochytrium aureum, Thraustochytrium roseum, Thraustochytrium striatum, or Schizochytrium sp.

7. The method of claim 1, wherein the oil producing microbe is from the family Thraustochytriaceae.

8. The method of claim 1, wherein the oil producing microbe is a microbe having ATCC Accession Number PTA-6245.

9. The method of claim 1, wherein the fatty acid production antagonist is oleic acid.

10. The method of claim 1, wherein the fatty acid production antagonist is selected from the group consisting of cerulenin, toluic acid, norflurazon, sethoxydim, and capsaicin.

11. The method of claim 1, wherein the fatty acid production antagonist is selected from the group consisting of curcumin, cyclopropene, alkyl gallate, propyl gallate, sesame oil, peanut oil, canola oil, pyridazinone, and diphenylamine.