Sample processing units, systems, and related methods

• US 9,023,655 B2
• Filed: 12/10/2013
• Issued: 05/05/2015
• Est. Priority Date: 09/16/2008
• Status: Expired due to Fees

• Current Assignee: Ibis Biosciences Incorporated (Abbott Laboratories)
• Original Assignee: Ibis Biosciences Incorporated (Abbott Laboratories)
• Inventors: Hofstadler, Steven A., Drader, Jared J., Gutierrez, Jose R., Gleason, Paul J., Bare, Rex O., Miller, Robert D., Smith, Jeffrey C.
• Primary Examiner(s): Mui, Christine T
• Application Number:
  US14/102,200
• Publication Number:
  US 20140224042A1
• Time in Patent Office: 511 Days
• Field of Search: 436/149, 436/150, 436/94, 436/93, 436/91, 422/63, 422/64, 422/65, 422/50
• US Class Current: 436/150
• CPC Class Codes: G01N 1/02 Devices for withdrawing samples sampling of foundation soil E02D1/04; collecting or conveying radioactive samples G01T7/00, e.g. G01T7/02, G01T7/08
• CPC Class Codes: G01N 1/10 in the liquid or fluent state burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00
• CPC Class Codes: G01N 2035/00524 Mixing by agitating sample carrier
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: Y10T 436/14 Heterocyclic carbon compound [i.e. , O, S, N, Se, Te, as only ring hetero atom]
• CPC Class Codes: Y10T 436/142222 Hetero-O [e.g., ascorbic acid, etc.]
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

Sample processing units useful for mixing and purifying materials, such as fluidic materials are provided. A sample processing unit typically includes a container configured to contain a sample comprising magnetically responsive particles, and one or more magnets that are in substantially fixed positions relative to the container. A sample processing unit also generally includes a conveyance mechanism configured to convey the container to and from a position that is within magnetic communication with the magnet, e.g., such that magnetically responsive particles with captured analytes can be retained within the container when other materials are added to and/or removed from the container. Further, a sample processing unit also typically includes a rotational mechanism that is configured to rotate the container, e.g., to effect mixing of sample materials disposed within the container. Related carrier mechanisms, sample processing stations, systems, and methods are also provided.

Claims

1. A sample processing unit, comprising:

at least one sample comprising at least one magnetically responsive particle;

at least one magnet that generates, or is configured to generate, at least one magnetic field, which magnet is in a substantially fixed position relative to the sample;

at least one conveyance mechanism configured to convey the sample between at least first and second positions, wherein at least the first position is within magnetic communication with the magnet when the magnet generates the magnetic field; and

,

at least one rotational mechanism, which rotational mechanism is configured to rotate the sample when the sample is in at least the second position.

2. The sample processing unit of claim 1, wherein the magnet comprises a permanent magnet.

3. The sample processing unit of claim 1, wherein the magnet comprises an electromagnet.

4. The sample processing unit of claim 1, wherein the conveyance mechanism comprises at least one motor.

5. The sample processing unit of claim 4, wherein at least one motor comprises a stepper motor.

6. The sample processing unit of claim 4, wherein at least one motor comprises a brushless direct current motor.

7. The sample processing unit of claim 1, wherein the conveyance mechanism is configured to rotate the sample between the first and second positions.

8. The sample processing unit of claim 1, wherein the conveyance mechanism is configured to slide the sample between the first and second positions.

9. The sample processing unit of claim 1, wherein the rotational mechanism is configured to rotate the sample in at least one oscillating motion.

10. The sample processing unit of claim 1, comprising at least one mounting bracket that is operably connected to one or more of:

the magnet, the conveyance mechanism, or the rotational mechanism.

11. the sample processing unit of claim 1, comprising a desalting station, a sample wash station, a fluid transfer station, or a mixing station.

12. The sample processing unit of claim 1, comprising at least one wash reagent.

13. The sample processing unit of claim 1, comprising at least one elution reagent.

14. The sample processing unit of claim 1, wherein the magnetically responsive particle comprises an anion exchange resin.

15. The sample processing unit of claim 14, wherein the magnetically responsive particle comprises at least one biopolymer capture reagent.

16. The sample processing unit of claim 15, wherein the biopolymer capture reagent comprises at least one anion exchange functional group.

17. The sample processing unit of claim 16, wherein the anion exchange functional group comprises a pK_a value of 9.0 or greater.

18. The sample processing unit of claim 16, wherein the anion exchange functional group is selected from the group consisting of a primary amine, a second amine, a tertiary amine, a quaternary amine, a polyethyleneimine, a charged aromatic amine, a diethylaminomethyl, and a diethylaminoethyl.

19. The sample processing unit of claim 1, wherein the sample processing unit comprises a controller comprising a programmed processor, a computer, a digital device, a logic device or an information appliance.